Choline Supplementation Normalizes Fetal Adiposity and Reduces Lipogenic Gene Expression in a Mouse Model of Maternal Obesity
Choline Supplementation Normalizes Fetal Adiposity and Reduces Lipogenic Gene Expression in a Mouse Model of Maternal Obesity
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DOI：
10.3390/nu9080899
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发表时间：
2017-08-01
期刊：
影响因子：
5.9
通讯作者：
Jiang, Xinyin
中科院分区：
文献类型：
--
作者：
Jack-Roberts, Chauntelle;Joselit, Yaelle;Jiang, Xinyin
母亲肥胖会增加胎儿的肥胖，这可能会对后代的代谢健康产生不利影响。胆碱调节脂质代谢，因此可能影响肥胖。本研究探讨了母体补充胆碱对母体肥胖小鼠模型胎儿肥胖的影响。C57 BL/6 J小鼠喂食高脂肪（HF）饮食或对照（NF）饮食，并在定时交配前和整个妊娠期间接受25 mM胆碱补充（CS）或对照未处理（CO）饮用水6周。在胚胎第17.5天，HF喂养导致HFCO组胎儿的总体脂百分比较高（p < 0.05），而补充胆碱的HFCS组与NFCO组相比没有显示出显著差异。类似地，HF喂养导致HFCO胎仔肝脏甘油三酯蓄积更高（p < 0.05），但HFCS胎仔没有。在HFCS组中，母体补充胆碱可下调脂肪生成基因（如Acc 1、Fads 1和Elovl 5）以及有利于脂肪生成的转录因子Srebp 1c的mRNA水平（p < 0.05），这可能是减少母体HF喂养期间胎儿肝脏脂肪蓄积的机制。总之，母体补充胆碱可改善妊娠晚期肥胖母鼠的胎儿肥胖指数。
Maternal obesity increases fetal adiposity which may adversely affect metabolic health of the offspring. Choline regulates lipid metabolism and thus may influence adiposity. This study investigates the effect of maternal choline supplementation on fetal adiposity in a mouse model of maternal obesity. C57BL/6J mice were fed either a high-fat (HF) diet or a control (NF) diet and received either 25 mM choline supplemented (CS) or control untreated (CO) drinking water for 6 weeks before timed-mating and throughout gestation. At embryonic day 17.5, HF feeding led to higher (p < 0.05) percent total body fat in fetuses from the HFCO group, while the choline supplemented HFCS group did not show significant difference versus the NFCO group. Similarly, HF feeding led to higher (p < 0.05) hepatic triglyceride accumulation in the HFCO but not the HFCS fetuses. mRNA levels of lipogenic genes such as Acc1, Fads1, and Elovl5, as well as the transcription factor Srebp1c that favors lipogenesis were downregulated (p < 0.05) by maternal choline supplementation in the HFCS group, which may serve as a mechanism to reduce fat accumulation in the fetal liver during maternal HF feeding. In summary, maternal choline supplementation improves indices of fetal adiposity in obese dams at late gestation.