DIFFERENTIAL REGULATION OF CLOSELY RELATED MEMBERS OF THE HSP16 GENE FAMILY IN CAENORHABDITIS-ELEGANS
DIFFERENTIAL REGULATION OF CLOSELY RELATED MEMBERS OF THE HSP16 GENE FAMILY IN CAENORHABDITIS-ELEGANS
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DOI：
10.1089/dna.1.1989.8.481
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发表时间：
1989-09-01
期刊：
影响因子：
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通讯作者：
CANDIDO, EPM
中科院分区：
文献类型：
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作者：
JONES, D;DIXON, DK;CANDIDO, EPM
秀丽隐杆线虫中编码16-kD热休克多肽的热诱导基因位于两个不同的基因座，一个含有16-1和16-48基因（基因座A），另一个含有16-2和16-41基因（基因座B）。尽管这些基因及其启动子的结构高度保守，但在热诱导过程中，B基因座产生的mRNA比A基因座多7倍。由于在A基因座有两个拷贝的16-1和16-48基因，mRNA产生的差异实际上在每个基因的基础上高达14：1。从热休克恢复过程中的热休克蛋白16 mRNA衰变率的测量表明，这种差异是不是由差异mRNA的稳定性，此外，核runon实验产生的转录率为16-1/48位点的约三倍高于那些从16-2/41位点。来自16-2/41位点的较高水平的mRNA，特别是在较长的诱导时间下，似乎是由于来自两个位点的mRNA产生的时间模式的显著差异。虽然两个基因座都被热休克瞬时激活，但A基因座的16-1和16-48基因比来自B基因座的基因更快地下调至较低的转录速率。
The heat-inducible genes encoding 16-kD heat shock polypeptides in Caenorhabditis elegans are found at two separate loci, one containing the 16-1 and 16-48 genes (locus A), and the other, the 16-2 and 16-41 genes (locus B). Despite the highly conserved structures of these genes and their promoters, the B locus produces up to sevenfold more mRNA during heat induction than does the A locus. Since there are two copies of the 16-1 and 16-48 genes at the A locus, the discrepancy in mRNA production is actually as high as 14:1 in a per gene basis. Measurements of the rate of hsp16 mRNA decay during recovery from a heat shock suggest that this difference is not caused by differential mRNA stability; furthermore, nuclear runon experiments yield rates of transcription for the 16-1/48 locus that are approximately threefold higher than those from the 16-2/41 locus. The higher levels of mRNA from the 16-2/41 locus, particularly at longer induction times, seem to be due to a marked difference in the temporal pattern of mRNA production from the two loci. While both loci are transiently activated by a heat shock, the 16-1 and 16-48 genes of the A locus are down-regulated to a lower transcription rate sooner than the genes from the B locus.