Multichannel microchip electrophoresis device fabricated in polycarbonate with an integrated contact conductivity sensor array
Multichannel microchip electrophoresis device fabricated in polycarbonate with an integrated contact conductivity sensor array
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DOI：
10.1021/ac0612168
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发表时间：
2007-02-01
影响因子：
7.4
通讯作者：
Soper, Steven A.
中科院分区：
文献类型：
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作者：
Shadpour, Hamed;Hupert, Mateusz L.;Soper, Steven A.
提出了一种具有集成接触式电导率传感器阵列的 16 通道微流控芯片。微流体网络由 16 个分离通道组成，使用高精度微铣削金属母模将其热压印到聚碳酸酯 (PC) 中。所有通道均为40μm深、60μm宽，有效分离长度为40mm。金 (Au) 传感器阵列通过光刻图案化到 PC 盖板上，并通过热粘合组装到流体芯片上，将一对 Au 微电极（60 μm 宽，5 μm 间距）合并到 16 个通道中的每个通道中，并用作独立的接触式电导率检测器。每个分离通道的相应流体储液器之间的间距设置为 9 毫米，这使得使用 8 通道移液器只需 5 个移液步骤即可将样品和缓冲液加载到位于微芯片上的所有 40 个储液器中。使用带有铂 (Pt) 线的印刷电路板 (PCB) 将电泳高压分配到位于流体芯片上的所有储液器。另一个 PCB 用于收集来自图案化金微电极的电导率信号。使用微芯片毛细管区带电泳 (mu-CZE) 对氨基酸、肽和蛋白质混合物以及通过微芯片毛细管电色谱 (mu-CEC) 分离的寡核苷酸评估装置性能。分离在 90 V/cm 的电场 (E) 下进行，并且在所有情况下均在 4 分钟内完成。使用双极脉冲电压波形进行电导率检测，脉冲幅度为+/-0.6V，频率为6.0kHz。电导率传感器阵列的丙氨酸检测浓度限（SNR = 3）确定为 7.1 μM。寡核苷酸的 mu-CEC 和氨基酸、肽和蛋白质的 mu-CZE 的分离效率分别为 6.4 x 10(4)、2.0 x 10(3)、4.8 x 10(3) 和 3.4 x 10(2) 板，平均通道间迁移时间重现性为 2.8%。寡核苷酸的 mu-CEC 和氨基酸、肽和蛋白质的 mu-CZE 获得的平均分辨率分别为 4.6、1.0、0.9 和 1.0。据我们所知，该报告是第一篇描述具有集成接触式电导率传感器阵列的多通道微芯片电泳装置的报告。
A 16-channel microfluidic chip with an integrated contact conductivity sensor array is presented. The microfluidic network consisted of 16 separation channels that were hot-embossed into polycarbonate (PC) using a high-precision micromilled metal master. All channels were 40 mu m deep and 60 mu m wide with an effective separation length of 40 mm. A gold (Au) sensor array was lithographically patterned onto a PC cover plate and assembled to the fluidic chip via thermal bonding in such a way that a pair of Au microelectrodes (60 mu m wide with a 5 mu m spacing) was incorporated into each of the 16 channels and served as independent contact conductivity detectors. The spacing between the corresponding fluidic reservoirs for each separation channel was set to 9 mm, which allowed for loading samples and buffers to all 40 reservoirs situated on the microchip in only five pipetting steps using an 8-channel pipettor. A printed circuit board (PCB) with platinum (Pt) wires was used to distribute the electrophoresis high-voltage to all reservoirs situated on the fluidic chip. Another PCB was used for collecting the conductivity signals from the patterned Au microelectrodes. The device performance was evaluated using microchip capillary zone electrophoresis (mu-CZE) of amino acid, peptide, and protein mixtures as well as oligonucleotides that were separated via microchip capillary electrochromatography (mu-CEC). The separations were performed with an electric field (E) of 90 V/cm and were completed in less than 4 min in all cases. The conductivity detection was carried out using a bipolar pulse voltage waveform with a pulse amplitude of +/- 0.6 V and a frequency of 6.0 kHz. The conductivity sensor array concentration limit of detection (SNR = 3) was determined to be 7.1 mu M for alanine. The separation efficiency was found to be 6.4 x 10(4), 2.0 x 10(3), 4.8 x 10(3), and 3.4 x 10(2) plates for the mu-CEC of the oligonucleotides and mu-CZE of the amino acids, peptides, and proteins, respectively, with an average channel-to-channel migration time reproducibility of 2.8%. The average resolution obtained for mu-CEC of the oligonucleotides and mu-CZE of the amino acids, peptides, and proteins was 4.6, 1.0, 0.9, and 1.0, respectively. To the best of our knowledge, this report is the first to describe a multichannel microchip electrophoresis device with integrated contact conductivity sensor array.