GENETIC-MAPPING AND VARIABILITY OF 7 SOYBEAN SIMPLE SEQUENCE REPEAT LOCI
GENETIC-MAPPING AND VARIABILITY OF 7 SOYBEAN SIMPLE SEQUENCE REPEAT LOCI
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DOI：
10.1139/g94-109
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发表时间：
1994-10-01
期刊：
影响因子：
3.1
通讯作者：
OLIVIERI, AM
中科院分区：
文献类型：
--
作者：
MORGANTE, M;RAFALSKI, A;OLIVIERI, AM
微卫星或简单重复序列是短串联重复的DNA序列基序的延伸，分散在大多数真核生物的基因组中。简单重复序列多态性（SSRPs）最近在植物中被报道。在此基础上，对7个大豆品种（Glycine max（L.）Merr.和大豆（Glycine soja Sieb.）Zucc）。SSRPs包含在测序的基因中，其中四个代表这些基因的新定位。另外三个SSRPs与独立建立的相应基因的RFLP图谱位置相吻合。当使用琼脂糖凝胶在这些位点中的四个位点上筛选一组61份大豆种质时，每个位点的平均等位基因数为7.75，有效等位基因数（n（e））为2.57，等位基因分化水平（delta（T））为0.62。随着驯化程度的提高，等位基因变异急剧下降，分化程度从野生大豆的84%下降到优良种质的43%。在61个品系中的19个品系组成的子集上观察到的SSRPs的变异水平总是高于RFLP标记，每个位点的等位基因平均数从4.25到2.15。与RFLP标记相比，SSRPs信息量更大，更容易分析，但需要更多的努力来开发。
Microsatellites or simple sequence repeats are stretches of short tandemly repeated DNA sequence motifs, dispersed throughout the genomes of most eukaryotes. Simple sequence repeat polymorphisms (SSRPs) have recently been reported in plants. Here we present the genetic map position of seven different soybean (Glycine max (L.) Merr. and Glycine soja Sieb. and Zucc.) SSRPs contained in sequenced genes, four of which represent newly mapped positions for these genes. The other three SSRPs coincided with independently established RFLP map positions for the corresponding genes. When a set of 61 soybean accessions was screened at four of these loci by using agarose gels, the average number of alleles per locus was 7.75, the effective number of alleles (n(e)) was 2.57, and the level of allele differentiation (delta(T)) was 0.62. Allelic variation decreased sharply with increasing levels of domestication, with the level of differentiation going from 84% in the wild soybean to 43% in the elite germplasm. Variation levels observed on a subset made of 19 of the 61 lines were always higher for SSRPs than for RFLP markers, with the average number of alleles per locus going from 4.25 to 2.15. In comparison with RFLP markers, SSRPs are more informative and are easier to analyse but require more effort to develop.