Short-term biological safety of a photoelectric dye used as a component of retinal prostheses
Short-term biological safety of a photoelectric dye used as a component of retinal prostheses
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DOI：
10.1007/s10047-008-0403-x
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发表时间：
2008-04-01
影响因子：
1.3
通讯作者：
Ohtsuki, Hiroshi
中科院分区：
文献类型：
--
作者：
Okamoto, Kazuo;Matsuo, Toshihiko;Ohtsuki, Hiroshi
我们设计了一种新型的视网膜假体，它带有一种光电染料，可以转移光子能量产生电势。本研究旨在测试用于视网膜假体的光电染料2-[2-[4-（二丁基氨基）苯基]乙烯基]-3-羧甲基溴化苯并噻唑鎓（NK-5962）的安全性。视网膜细胞来源于胚胎期12天的鸡的感觉神经视网膜，是视网膜神经元和神经胶质细胞的混合群体，并且在避光或在不同浓度的光电染料存在下在230勒克斯每天9小时的连续光照下培养2天（1.6 x 10（-5）、1.6 x 10（-6）和1.6 x 10（-7）M），通过对活细胞和死细胞染色来评估细胞活力。在避光条件下或在320 lux连续光照条件下，用不同浓度（6.6 x 10（-5）、6.6 x 10（-6）和6.6 x 10（-）7 M）的光电染料孵育相同胚胎期的分散视网膜色素上皮细胞4 h，以通过测量细胞泄漏的乳酸脱氢酶活性来评估细胞毒性。在存在或不存在光电染料的黑暗条件或光照条件下，大多数视网膜细胞是活的，只有一小部分死细胞。在较高浓度的光电染料下，死细胞的百分比显著较小（P = 0.0183，双因素方差分析），而在黑暗条件和光照条件下，死细胞的百分比没有显著差异（P = 0.3102）。细胞毒性百分比值为阴性，表明在用不同浓度的光电染料孵育的所有视网膜色素上皮细胞组中具有保护作用。该光电染料短期暴露对鸡视网膜细胞和视网膜色素上皮细胞无细胞毒性。此外，这种光电染料可能对两种类型的细胞都有保护作用。
We have designed a new type of retinal prosthesis with a photoelectric dye that transfers photon energy to generate electric potentials. The purpose of this study was to test the safety of a photoelectric dye, 2-[2-[4-(dibutylami no)phenyl]ethenyl]-3-carboxymethylbenzothiazolium bromide (NK-5962), used for retinal prostheses. The retinal cells, derived from chick neurosensory retinas at the 12-day embryonic stage, were a mixed population of retinal neurons and glial cells, and were cultured for 2 days either under protection from light or under continuous light exposure at 230 lux for 9 h daily in the presence of the photoelectric dye at varying concentrations (1.6 x 10(-5), 1.6 x 10(-6), and 1.6 x 10(-7) M) to assess cell viability by staining live cells and dead cells. Dispersed retinal pigment epithelial cells at the same embryonic stage were incubated with the photoelectric dye at varying concentrations (6.6 x 10(-5), 6.6 x 10(-6), and 6.6 x 10(-)7 M) for 4 h under protection from light or under continuous light exposure at 320 lux to assess cytotoxicity by measuring the activity of lactate dehydrogenase leaking from cells. The majority of retinal cells were alive with only a small percentage of dead cells under the dark condition or the light condition in the presence or the absence of the photoelectric dye. The percentage of dead cells was significantly smaller at higher concentrations of the photoelectric dye (P = 0.0183, two-factor analysis of variance), while the percentage of dead cells was not significantly different between the dark condition and the light condition (P = 0.3102). Percent cytotoxicity values were negative, indicating protective effects in all groups of retinal pigment epithelial cells incubated with varying concentrations of the photoelectric dye. The photoelectric dye showed no cytotoxicity to chick retinal cells or retinal pigment epithelial cells on short-term exposure. In addition, this photoelectric dye might have protective effects on both types of cells.