STRUCTURAL STUDY ON THE CARBOHYDRATE MOIETY OF HUMAN PLACENTAL ALKALINE-PHOSPHATASE
STRUCTURAL STUDY ON THE CARBOHYDRATE MOIETY OF HUMAN PLACENTAL ALKALINE-PHOSPHATASE
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DOI：
10.1093/oxfordjournals.jbchem.a122228
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发表时间：
1988-01-01
影响因子：
2.7
通讯作者：
KOBATA, A
中科院分区：
文献类型：
--
作者：
ENDO, T;OHBAYASHI, H;KOBATA, A
从人胎盘中纯化的碱性磷酸酶在一个分子中含有单个天冬酰胺连接的糖链。通过肼解，然后通过N-乙酰化和NaB 3 H4还原，将糖链从多肽部分定量释放为放射性寡糖，并通过纸电泳分离成一个中性和两个酸性部分。通过连续外切糖苷酶消化和甲基化酸性组分的组合。通过连续的外切糖苷酶消化和甲基化分析的组合，证实中性级分中寡糖的结构如下：Gal β 1 → 4GlcNAc β 1 → 2 Man α 1 → 6（Gal β 1 → 4GlcNAc β 1 → 2 Man α 1 → 3）Man β 1 → 4GlcNAc β 1 → 4（±）。Fuca 1 → 6）GlcNAc。酸性寡糖级分是中性级分的单唾液酸衍生物和二唾液酸衍生物的混合物。糖链的所有唾液酸残基以NeuAc α 2 → 3Gal基团存在。在单唾液酸衍生物的情况下，N-乙酰神经氨酸仅与Man α 1 → 3臂连接。
Alkaline phosphatase purified from human placenta contains a single asparagine-linked sugar chain in one molecule. The sugar chain was quantitatively liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and NaB3H4 reduction, and separated by paper electrophoresis into one neutral and two acidic fractions. By a combination of sequential exoglycosidase digestion and methylation acidic fractions. By a combination of sequential exoglycosidase digestion and methylation analysis, the structures of oligosaccharides in the neutral fraction were confirmed to be as follows: Gal.beta.1.fwdarw.4GlcNAc .beta.1.fwdarw.2Man.alpha.1.fwdarw.6(Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.2Man.alpha.1.fwdarw.3)Man.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.4(.+-.Fuc.alpha.1.fwdarw.6)GlcNAc. The acidic oligosaccharide fractions were mixtures of mono- and disialyl derivatives of the neutral fraction. All the sialic acid residues of the sugar chains occur as the NeuAc.alpha.2.fwdarw.3Gal group. In the case of monosialyl derivatives, the N-acetylneuraminic acid was exclusively linked to the Man.alpha.1.fwdarw.3 arm.