Identification for Heat Tolerance in Backcross Recombinant Lines and Screening of Backcross Introgression Lines with Heat Tolerance at Milky Stage in Rice
Identification for Heat Tolerance in Backcross Recombinant Lines and Screening of Backcross Introgression Lines with Heat Tolerance at Milky Stage in Rice
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DOI：
10.1016/s1672-6308(12)60006-7
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发表时间：
2011-12-01
期刊：
影响因子：
4.8
通讯作者：
Huang Ying-jin
中科院分区：
文献类型：
--
作者：
Liao Jiang-lin;Zhang Hong-yu;Huang Ying-jin
本研究旨在选育基因组相似但灌浆期耐热性显著差异的水稻新种质。以谢青藻B / N-22 //谢青藻B杂交获得的791个BC1F8回交重组系为材料。将每个水稻品系平均分为两组，在田间自然高温条件下评价所有水稻品系的耐热性。选择耐热指数高于90%或低于40%的水稻品系进行表型性状比较，进一步鉴定植园乳白期早期的耐热性。利用均匀分布在12条水稻染色体上的887个简单序列重复标记，对表型性状相似但乳白期耐热性差异显著的水稻品系进行了分析。结果得到12对（6对）表型性状相近但乳白期耐热性差异显著的水稻品系。分子标记分析表明，703T和704S在6对水稻品系中多态性最小，多态性位点只有16个，包含22个不同的等位基因。利用这两个回交渐渗水稻品系进行水稻乳浆期耐热机制的研究，理论上有利于减少材料遗传差异的干扰。
The present study aimed at breeding new rice germplasms with similar genome but significantly differed in heat tolerance during the grain filling stage. A total of 791 BC1F8 backcross recombinant lines, derived from the cross of Xieqingzao B / N-22 // Xieqingzao B, were used as materials. Each rice line was separated evenly into two groups, and the heat tolerance of all rice lines were evaluated at natural high temperature in fields. The rice lines with heat tolerant index higher than 90% or lower than 40% were selected to compare the phenotypic characters and further identify heat tolerance at the early milky stage in a phytotron. Rice lines with similar phenotypic characters but significantly differed in heat tolerance at the milky stage were analyzed by 887 simple sequence repeat markers that were evenly distributed on the 12 rice chromosomes. In the result, 12 (6 pairs) rice lines with similar phenotypic characters but significantly differed in heat tolerance at the milky stage were obtained. Molecular marker analysis indicated that the genomic polymorphism between 703T and 704S was the smallest in the 6 pairs of rice lines, with only 16 polymorphic sites, including 22 different alleles. The application of these two backcross introgression rice lines for future study on the mechanisms of heat tolerance in rice at the milky stage will be theoretically beneficial in reducing the interference caused by genetic differences from experimental materials.