Drug-releasing scaffolds fabricated from drug-loaded microspheres
Drug-releasing scaffolds fabricated from drug-loaded microspheres
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DOI：
10.1002/jbm.1251
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发表时间：
2002-02-01
期刊：
影响因子：
--
通讯作者：
Shea, LD
中科院分区：
文献类型：
--
作者：
Nof, M;Shea, LD
生物可降解支架在许多组织工程替代或引导组织再生的策略中发挥着核心作用，通常这些支架的功能是创造和维持空间，并为细胞黏附提供支持。然而，这些支架也可以作为运送生物活性因子(如蛋白质或DNA)的载体，以操纵支架微环境中的细胞过程。这项研究提出了一种新的方法来制造能够持续药物输送的组织工程支架，通过将载药微球制造成具有可控孔隙率的结构。采用复乳法制备了包裹有完整DNA的微球，并在24 h内从微球中释放出来，然后通过气体发泡工艺将其制成无孔圆盘或相互连接的开孔支架(>；94%孔隙率)。这种将载药微球组装成多孔性和无孔性结构的方法在合成基质的制备中具有很大的应用价值，可以指导组织形成。(C)2001年John Wiley&Sons，Inc.
Biodegradable scaffolds serve a central role in many strategies for engineering tissue replacements or in guiding tissue regeneration, Typically, these scaffolds function to create and maintain a space and to provide a support for cell adhesion. However, these scaffolds also can serve as vehicles for the delivery of bioactive factors (e.g., protein or DNA) in order to manipulate cellular processes within the scaffold microenvironment. This study presents a novel approach to fabricate tissue-engineering scaffolds capable of sustained drug delivery whereby drug-loaded microspheres are fabricated into structures with controlled porosity. A double-emulsion process was used to fabricate microspheres with encapsulated DNA that retained its integrity and was released from the microspheres within 24 h. These DNA-loaded microspheres subsequently were formed into a nonporous disk or an interconnected open-pore scaffold (> 94% porosity) via a gas-foaming process. The disks and scaffolds exhibited sustained plasmid release for at least 21 days and had minimal burst during the initial phase of release This approach of assembling drug-loaded microspheres into Porous and nonporous structures may find great utility in the fabrication of synthetic matrices that direct tissue formation. (C) 2001 John Wiley & Sons, Inc.