A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo
A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo
复制标题
一种独特的开关近红外花青探针，用于细胞和体内内源性碱性磷酸酶活性成像
DOI：
10.1016/j.snb.2018.03.078
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chen Qingguo
中科院分区：
文献类型：
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作者：
Gao Zhiwei;Sun Jingya;Gao Min;Yu Fabiao;Chen Lingxin;Chen Qingguo
碱性磷酸酶（ALP）是一类广泛存在于人体各种组织中的酶。它在调节多种细胞功能中起着重要作用。血清ALP的异常水平与疾病有关。开发一种灵敏、准确的评价生物体内ALP水平变化的检测工具，将对生物化学、细胞学和临床医学等领域有很大的帮助。在此，我们开发了一种近红外探针（QcyP），用于特异性检测不同细胞系和荷瘤小鼠模型中不同ALP水平。探针由两个部分组成：一个独特的七甲基菁荧光团和一个磷酸单酯。ALP可以通过酶催化的磷酸基裂解触发荧光开关。荧光调节机制是基于共轭π-电子系统的重排。该探针对碱性磷酸酶具有高选择性和良好的灵敏度。结果表明，QcyP探针不仅可以用于区分不同细胞系的ALP水平，而且可以区分肝肿瘤小鼠模型与正常小鼠之间ALP水平的变化。
Alkaline phosphatase (ALP) is a class of enzymes that are widely found in various tissues of the human body. It plays important roles in regulating diverse cellular functions. The aberrant levels of serum ALP are implicated in diseases. The development of sensitive and accurate detection tool for evaluating the level changes of ALP in living organisms will be very helpful in the fields of biochemistry, cytology and clinical medicine. Herein, we develop a near-infrared probe (QcyP) for the specific detection of different ALP levels both in different cell lines and in tumor-bearing mice models. The probe is composed of two moieties: a unique heptamethine cyanine fluorophore and a phosphate monoester. ALP can trigger an off-on fluorescence switch via an enzyme-catalyzed cleavage of the phosphate group. The fluorescence regulation mechanism is based on the rearrangement of the conjugated π-electron system. The probe exhibits high selectivity and good sensitivity towards ALP. The results demonstrate that the probe QcyP not only can be applied to discriminate the levels of ALP in different cell lines, but also can distinguish the ALP level changes between hepatic tumor mice model and normal mice.