A sensitive assay for simultaneous determination of plasma concentrations of valganciclovir and its active metabolite ganciclovir by LC/MS/MS
A sensitive assay for simultaneous determination of plasma concentrations of valganciclovir and its active metabolite ganciclovir by LC/MS/MS
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DOI：
10.1016/j.jchromb.2006.10.053
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发表时间：
2007-04-01
影响因子：
3
通讯作者：
Yu, Chen
中科院分区：
文献类型：
--
作者：
Xu, Hong-Rong;Li, Xue-Ning;Yu, Chen
建立了同时测定人血浆中伐更昔洛韦及其活性代谢物更昔洛韦的蛋白质沉淀-高效液相色谱-串联质谱法(LC/MS/MS)。该溶剂体系还可作为蛋白质沉淀剂。色谱柱为Aquasil C18柱(50 mm×2.1 mm，5µm)。采用0.02%甲酸-甲醇的线性梯度流动相。检测采用阳离子电喷雾串联质谱仪，色谱柱为Sciex API3000。万乃昔洛韦为4~512 ng/m L，更昔洛韦为0.1~12.8 m u g/m L，符合1/x加权二次回归模型。该方法准确、特异、灵敏，已成功应用于药代动力学研究。(C)2006爱思唯尔B.V.保留所有权利。
A protein precipitation, liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the simultaneous determination of valganciclovir and its active metabolite ganciclovir in human plasma. The solvent system also served as a protein precipitation reagent. The chromatographic separation was achieved on an Aquasil C18 column (50mm x 2.1 mm, 5 mu m). A linear gradient mobile phase between 0.02% formic acid and methanol was used. Detection was by positive ion electrospray tandem mass spectrometry on a Sciex API3000. The standard curves, which ranged from 4 to 512 ng/mL for valganciclovir and from 0.1 to 12.8 mu g/mL for ganciclovir, were fitted to a 1/x weighted quadratic regression model. The method was proved to be accurate, specific and sensitive enough and was successfully applied to a pharmacokinetic study. (c) 2006 Elsevier B.V. All rights reserved.