Biochemical changes in the developing rat central nervous system due to hyperthermia.
Biochemical changes in the developing rat central nervous system due to hyperthermia.
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由于高温导致发育中的大鼠中枢神经系统发生生化变化。
DOI：
10.1002/tera.1420410310
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Ziskin,MC
中科院分区：
文献类型：
--
作者：
Rao,GS;Abraham,V;Fink,BA;Margulies,N;Ziskin,MC
将妊娠 10 天的大鼠胚胎暴露在 43°C 下 8 分钟，将取出的右子宫角浸入加热的盐水溶液中，然后将子宫角重新插入腹腔。第 15 天时，取出胎儿，分离大脑半球的细胞并作为原代培养物生长。来自左子宫角的胚胎作为对照。在不同培养天数的对照胚胎和热暴露胚胎的细胞培养物之间没有观察到形态变化。然而，胚胎在第 10 天暴露于高温会显着影响与胆碱能神经元相关的乙酰胆碱酯酶以及与少突胶质细胞和髓磷脂膜形成相关的 2',3'-环核苷酸磷酸水解酶的活性发育模式。这些结果表明，大鼠妊娠 10 天时的高温可能会导致中枢神经系统神经元和少突胶质细胞发育受损。
Rat embryos at 10 days of gestation were exposed to 43°C for 8 minutes by submerging the exteriorized right uterine horn in heated saline solution and then reinserting the uterine horn into the abdominal cavity. At 15 days, the fetuses were removed, and cells from the cerebral hemispheres were dissociated and grown as primary cultures. Embryos from the left uterine horn served as controls. No morphological changes were observed between the cultures of cells from control and heat‐exposed embryos at different days in culture. However, exposure of embryos to hyperthermia at 10 days significantly affected the developmental pattern of activities of acetylcholine esterase associated with cholinergic neurons and of 2′,3′‐cyclic nucleotide phosphohydrolase associated with oligodendrocytes and myelin membrane formation. These results suggest that hyperthermia at 10 days of gestation in the rat may lead to an impairment in the development of neurons and oligodendrocytes in the central nervous system.