DECOMPOSITION OF PHOSPHOSERINE AND PHOSPHOTHREONINE DURING ACID-HYDROLYSIS
DECOMPOSITION OF PHOSPHOSERINE AND PHOSPHOTHREONINE DURING ACID-HYDROLYSIS
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DOI：
10.1016/0003-2697(76)90197-4
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发表时间：
1976-01-01
影响因子：
2.9
通讯作者：
HUANG, TS
中科院分区：
文献类型：
--
作者：
BYLUND, DB;HUANG, TS
研究了在110℃条件下，用1、2和6盐酸进行酸水解时，作为游离o -磷氨基酸和多肽的磷丝氨酸和磷苏氨酸的分解速率。C.对于游离的o -磷氨基酸，分解遵循一级动力学，磷丝氨酸的分解速度比磷苏氨酸快2- 4倍。在肽水解过程中，这些o -磷氨基酸的破坏速率取决于邻近的氨基酸残基，因此游离o -磷氨基酸的水解通常不是肽中o -磷氨基酸水解的良好模型。对于这3种肽，在6盐酸中水解2-4小时后，o -磷氨基酸的回收率最高。
The rate of decomposition of phosphoserine and phosphothreonine, both as free O-phosphoamino acids and in peptides, was studied under conditions of acid hydrolysis, using 1, 2 and 6 N HCl at 110.degree. C. For the free O-phosphoamino acids the decomposition follows first-order kinetics and is 2- to 4-fold faster for phosphoserine than phosphothreonine. The rate of destruction of these O-phosphoamino acids during hydrolysis of peptides is dependent on the neighboring amino acid residues, and thus the hydrolysis of a free O-phosphoamino acid generally is not a good model for the hydrolysis of that O-phosphoamino acids in a peptide. For the 3 peptides, maximal recoveries of O-phosphoamino acids are obtained after hydrolysis in 6 N HCl for 2-4 h.