Comparing transcriptome profiles of human embryo cultured in closed and standard incubators
Comparing transcriptome profiles of human embryo cultured in closed and standard incubators
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DOI：
10.7717/peerj.9738
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发表时间：
2020-08-11
期刊：
影响因子：
2.7
通讯作者：
Huang, Guoning
中科院分区：
文献类型：
--
作者：
Li, Jingyu;Huang, Jiayu;Huang, Guoning
有必要比较在延时成像（TLI）培养箱和标准培养箱（SI）中培养的人类胚胎的转录组学特征，以确定封闭培养系统是否对胚胎产生积极影响。在这项研究中，我们使用RNA测序（RNA-Seq）来表征和比较在TLI和SI中培养的相同质量等级的八细胞胚胎的基因表达谱。我们对受精卵、TLI培养的和SI培养的八细胞胚胎的总共580，952，620个读数进行了测序。TLI胚胎的总体转录组学特征与SI胚胎的相似，并且与合子高度不同。我们还检测到539个基因在TLI和SI组之间显示差异表达，其中错误发现率（FDR）< 0.05。通过基因本体富集分析，我们发现高表达的SI基因倾向于执行转录、RNA剪接和DNA修复等功能，而高表达的TLI基因富集在细胞分化和甲基转移酶活性途径中。这项研究是第一个使用转录组分析比较SI和TLI的研究，将作为评估TLI在辅助生殖技术中应用的安全性的基础。
It is necessary to compare the transcriptomic profiles of human embryos cultured in time-lapse imaging (TLI) incubators and standard incubators (SI) in order to determine whether a closed culture system has a positive impact on embryos. In this study, we used RNA-sequencing (RNA-Seq) to characterize and compare the gene expression profiles of eight-cell embryos of the same quality grade cultured in TLI and SI. We sequenced a total of 580,952,620 reads for zygotes, TLI-cultured, and SI-cultured eight-cell embryos. The global transcriptomic profiles of the TLI embryos were similar to those of the SI embryos and were highly distinct from the zygotes. We also detected 539 genes showing differential expression between the TLI and SI groups with a false discovery rate (FDR) < 0.05. Using gene ontology enrichment analysis, we found that the highly expressed SI genes tended to execute functions such as transcription, RNA splicing, and DNA repair, and that the highly expressed TLI genes were enriched in the cell differentiation and methyltransferase activity pathways. This study, the first to use transcriptome analysis to compare SI and TLI, will serve as a basis for assessing the safety of TLI application in assisted reproductive technology.