Overexpression of IbSINA5 Increases Cold Tolerance through a CBF SINA-COR Mediated Module in Sweet Potato
Overexpression of IbSINA5 Increases Cold Tolerance through a CBF SINA-COR Mediated Module in Sweet Potato
复制标题
IbSINA5 的过表达通过 CBF SINA-COR 介导的模块提高甘薯的耐冷性
DOI：
10.32604/phyton.2021.016314
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发表时间：
2021-01-01
影响因子：
1.7
通讯作者：
Zhou, Shenglin
中科院分区：
文献类型：
--
作者：
Li, Shiyang;Liu, Xue-Ao;Zhou, Shenglin
SINA家族蛋白在植物的生长发育和抗非生物胁迫中起着重要作用。然而，它们在植物对冷胁迫的反应中的生物学功能在很大程度上仍然未知。本研究从甘薯中分离到一个7基因IbSINA 5。实时荧光定量PCR（qRT-PCR）分析表明，IbSINA 5基因在甘薯各组织器官中广泛表达，在须根中表达量最高，且在低温、干旱和盐胁迫下表达量显著增加。亚细胞定位实验表明，IbSINA 5-GFP融合蛋白主要定位于细胞质和细胞核。IbSINA 5基因在甘薯中的过量表达显著提高了转基因植株对冷胁迫的抗性，这与IbCOR（cold-regulated）基因的表达上调、脯氨酸产量增加、丙二醛（MDA）和H2 O2积累减少有关。此外，IbCBF 3，一个C-重复结合因子（CBF）基因，在野生型甘薯植物的叶原生质体瞬时表达上调IbSINA 5和IbCOR基因的表达。研究结果表明，IbSINA 5可能通过CBF-SINA-COR介导的模块在甘薯冷信号转导途径中发挥正调控作用，具有很大的潜力，可作为候选基因用于未来培育抗寒性更强的植物新品种。
Seven in absentia (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a seven in absentia gene IbSINA5 was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that IbSINA5 was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm and nucleus. Overexpression of IbSINA5 in sweet potato led to dramatically improved resistance to cold stress in transgenic plants, which was associated with the up-regulated expression of IbCOR (cold-regulated) genes, increased proline production, and decreased malondialdehyde (MDA) and H2O2 accumulation in the leaves of transgenic plants. Furthermore, transient expression of IbCBF3, a C-repeat binding factor (CBF) gene, in the leaf protoplasts of wild type sweet potato plants up-regulated the expression of both IbSINA5 and IbCOR genes. Our results suggest that IbSINA5 could function as a positive regulator in the cold signaling pathway through a CBF-SINA-COR mediated module in sweet potato, and have a great potential to be used as a candidate gene for the future breeding of new plant species with improved cold resistance.