Cadherin interplay during neural crest segregation from the non-neural ectoderm and neural tube in the early chick embryo
Cadherin interplay during neural crest segregation from the non-neural ectoderm and neural tube in the early chick embryo
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DOI：
10.1002/dvdy.24517
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发表时间：
2017-07-01
影响因子：
2.5
通讯作者：
Duband, Jean-Loup
中科院分区：
文献类型：
--
作者：
Dady, Alwyn;Duband, Jean-Loup
背景：在鸟类胚胎中，神经嵴（NC）祖细胞在原肠胚形成期间出现在神经外胚层中，早在它们传播之前。虽然参与NC规范的基因调控网络已被破译，其隔离从其他神经外胚层衍生的祖细胞，特别是表皮和神经管，所涉及的机制是未知的。由于钙粘蛋白介导细胞识别和分选，我们仔细检查他们的表达谱在NC规范和分层。结果如下：我们发现，NC领土的定义早熟的强大的表达钙粘蛋白-6B的细胞中最初分散在其他细胞均匀表达E-钙粘蛋白，NC祖细胞逐步分选和重组成一个离散的域之间的前景表皮和神经管。在完成NC规格，表皮，NC和神经管完全隔离在连续的车厢，其特征在于不同的钙粘蛋白库。我们还发现，钙粘蛋白-6B的下调构成了一个重大事件，在NC分层，除了尾部的胚胎，N-钙粘蛋白是不太可能控制NC移民。结论：我们的研究结果表明，分区的神经外胚层介导的钙粘蛋白的相互作用，并归因于一个关键的作用，钙粘蛋白-6B的规范和分层的NC人口。发展动力学246：550-565，2017年。(c)2017年Wiley Periodicals，Inc.
Background: In the avian embryo, neural crest (NC) progenitors arise in the neuroectoderm during gastrulation, long before their dissemination. Although the gene regulatory network involved in NC specification has been deciphered, the mechanisms involved in their segregation from the other neuroectoderm-derived progenitors, notably the epidermis and neural tube, are unknown. Because cadherins mediate cell recognition and sorting, we scrutinized their expression profiles during NC specification and delamination. Results: We found that the NC territory is defined precociously by the robust expression of Cadherin-6B in cells initially scattered among other cells uniformly expressing E-cadherin, and that NC progenitors are progressively sorted and regrouped into a discrete domain between the prospective epidermis and neural tube. At completion of NC specification, the epidermis, NC, and neural tube are fully segregated in contiguous compartments characterized by distinct cadherin repertoires. We also found that Cadherin-6B down-regulation constitutes a major event during NC delamination and that, with the exception of the caudal part of the embryo, N-cadherin is unlikely to control NC emigration. Conclusions: Our results indicate that partition of the neuroectoderm is mediated by cadherin interplays and ascribes a key role to Cadherin-6B in the specification and delamination of the NC population. Developmental Dynamics 246:550-565, 2017. (c) 2017 Wiley Periodicals, Inc.