Functional parameters of voltage-activated Ca2+ currents from olfactory interneurons in the antennal lobe of Periplaneta americana.
Functional parameters of voltage-activated Ca2+ currents from olfactory interneurons in the antennal lobe of Periplaneta americana.
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美洲大蠊触角叶嗅觉中间神经元电压激活 Ca2 电流的功能参数。
DOI：
10.1152/jn.00719.2007
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发表时间：
2008
影响因子：
2.5
通讯作者：
P. Kloppenburg
中科院分区：
文献类型：
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作者：
A. Husch;Simon Hess;P. Kloppenburg
为了更好地了解介导细胞水平嗅觉信息处理的生理参数，我们在两种条件下分析了成年蟑螂触角叶嗅觉中间神经元的电压激活钙电流（I（Ca））：1）在急性分离的细胞中（体外）和2）在完整的大脑制备物中（原位）。该研究包括对潜在无机和有机 Ca(2+) 通道阻滞剂的调节作用的分析。使用药理学、电压和离子替代方案分离并鉴定 I(Ca)。 I(Ca) 由两部分组成：瞬态和持续。瞬态分量的衰减很大程度上依赖于Ca(2+)。在体外，I(Ca) 的激活阈值为 -50 mV，最大峰值电流为 -7 mV，尾电流激活的半最大电压 (V(0.5act)) 为 -18 mV。在原位，这些参数显着转移到更去极化的膜电位：I(Ca) 在 -40 mV 下激活，最大峰值电流为 8 mV，尾电流激活的 V(0.5act) 为 -11 mV。 I(Ca)对二价阳离子Cd(2+)、Co(2+)和Ni(2+)的敏感性呈剂量依赖性。最有效的阻断剂是Cd(2+)，IC(50)为10(-5) M，其次是Ni(2+)(IC(50)=3.13 x 10(-3) M)和Co(2+)(IC(50)=1.06 x 10(-3) M)。有机通道阻滞剂维拉帕米、地尔硫卓和硝苯地平也以剂量依赖性方式阻断 I(Ca)，并对电流波形产生不同的影响。维拉帕米阻断 I(Ca) 的 IC(50) 为 1.5 x 10(-4) M，地尔硫卓的 IC(50) 为 2.87 x 10(-4) M。硝苯地平在浓度为 10(-4) M 时阻断 I(Ca) 33%。
Toward our goal to better understand the physiological parameters that mediate olfactory information processing on the cellular level, voltage-activated calcium currents (I(Ca)) in olfactory interneurons of the antennal lobe from adult cockroaches were analyzed under two conditions: 1) in acutely dissociated cells (in vitro) and 2) in an intact brain preparation (in situ). The study included an analysis of modulatory effects of potential inorganic and organic Ca(2+) channel blockers. I(Ca) was isolated and identified using pharmacological, voltage, and ion substitution protocols. I(Ca) consisted of two components: transient and sustained. The decay of the transient component was largely Ca(2+) dependent. In vitro, I(Ca) had an activation threshold of -50 mV with a maximal peak current at -7 mV and a half-maximal voltage (V(0.5act)) for tail-current activation of -18 mV. In situ these parameters were significantly shifted to more depolarized membrane potentials: I(Ca) activated at -40 mV with a maximal peak current at 8 mV and a V(0.5act) for tail-current activation of -11 mV. The sensitivity of I(Ca) to the divalent cations Cd(2+), Co(2+), and Ni(2+) was dose dependent. The most effective blocker was Cd(2+) with an IC(50) of 10(-5) M followed by Ni(2+) (IC(50)=3.13 x 10(-3) M) and Co(2+) (IC(50)=1.06 x 10(-3) M). The organic channel blockers verapamil, diltiazem, and nifedipine also blocked I(Ca) in a dose-dependent way and had differential effects on the current waveform. Verapamil blocked I(Ca) with an IC(50) of 1.5 x 10(-4) M and diltiazem had an IC(50) of 2.87 x 10(-4) M. Nifedipine blocked I(Ca) by 33% at a concentration of 10(-4) M.
C.F.Wu、K.Sasaki、Y.Hotta：“从胞质分裂抑制的胚胎神经母细胞分化而来的巨型果蝇神经元。”
DOI：
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DOI：
10.14740/jocmr3671
发表时间：
2019-01-01
期刊：
Journal of clinical medicine research
影响因子：
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作者：
Yamamoto, Tomohiko;Miura, Shin-Ichiro;Urata, Hidenori
通讯作者：
Urata, Hidenori