Expression of a transcription factor from Capsicum annuum in pine calli counteracts the inhibitory effects of salt stress on adventitious shoot formation
Expression of a transcription factor from Capsicum annuum in pine calli counteracts the inhibitory effects of salt stress on adventitious shoot formation
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DOI：
10.1007/s00438-006-0137-5
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
Charles, Thomas M.
中科院分区：
文献类型：
--
作者：
Tang, Wei;Newton, Ronald J.;Charles, Thomas M.
调节应激反应基因表达的转录因子在植物的逆境适应中起着重要作用。在本研究中，我们检测了过表达辣椒乙烯响应因子/AP2型转录因子（CaPF1）的转基因弗吉尼亚松（Pinus virginiana Mill.）的耐盐胁迫能力，该转录因子可能增强转基因植物在营养生长过程中耐受各种胁迫的能力。CaPF1转基因提高了松树组织的耐盐和抗氧化胁迫能力，并抵消了盐胁迫对转基因弗吉尼亚松愈伤组织、枝条和植株生长的抑制作用。令我们惊讶的是，在不同氯化钠浓度胁迫下，三个CaPF1转基因弗吉尼亚松细胞系的枝条形成能力增强。200 mM的氯化钠显著提高了不定芽形成的频率以及每克愈伤组织的芽数。植物激素测定表明，与对照相比，CaPF1过表达的弗吉尼亚松愈伤组织中细胞分裂素水平发生了改变。根据我们的研究结果，我们推测细胞分裂素水平的改变可能通过一种未知机制导致暴露于盐中1周的转基因愈伤组织不定芽形成增强。
Transcription factors regulating the stress-responsive gene expression play an important role in plant stress adaptation. In this study, we examined the salt stress tolerance of transgenic Virginia pine (Pinus virginiana Mill.) overexpressing a Capsicum annuum ERF/AP2-type transcription factor (CaPF1), which may enhance the ability of transgenic plants to tolerate various kinds of stresses during vegetative growth. CaPF1 transgene increased the salt and oxidative stress tolerances of pine tissues and counteracted the inhibitory effects of salt stress on the growth of transgenic Virginia pine calli, shoots, and plants. To our surprise, the ability of shoot formation was enhanced in three CaPF1 transgenic Virginia pine cell lines under stress of different NaCl concentrations. NaCl at 200 mM significantly increased the frequency of adventitious shoot formation and the number of shoots per gram calli. Measurement of plant hormone demonstrated that the levels of cytokinin was altered in CaPF1-overexpressed Virginia pine calli, compared to the control. Based on our results, we speculate that the altered level of cytokinin may result in enhancing adventitious shoot formation of transgenic calli exposed to salt for 1 week via an unknown mechanism.