Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation.
Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation.
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DOI：
10.21053/ceo.2018.00486
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发表时间：
2019-03
影响因子：
3
通讯作者：
Cansiz H
中科院分区：
文献类型：
--
作者：
Batioglu-Karaaltin A;Ovali E;Karaaltin MV;Yener M;Yılmaz M;Eyüpoğlu F;Yılmaz YZ;Bozkurt ER;Demir N;Konuk E;Bozdağ ES;Yiğit Ö;Cansiz H
本研究的目的是利用物理、化学和酶的结合技术来缩短气管的脱细胞时间。取42只新西兰兔（3.5±0.5 kg）约3.5 cm长的气管段，按照脱细胞方案分为7组。脱细胞后，对支架的细胞区域、基质、强度和耐力进行随访。在50 ng/mg条件下测定各组DNA含量，冻干+脱氧胆酸+脱氧核糖核酸酶法3组的糖胺聚糖含量与对照组无显著差异（P=0.46）。脱细胞组与正常气管的拉应力值无显著差异（P < 0.05）。所有脱细胞组的上清液葡萄糖消耗量和乳酸水平与仅细胞组（76 mg/dL和53 mg/L）接近。使用组合方法可以减少化学物质的暴露，防止基质的过度影响，缩短脱细胞时间。
The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up. DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L). Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time.