Interactions of Nitrosomonas europaea and Nitrobacter winogradskyi grown in co-culture
Interactions of Nitrosomonas europaea and Nitrobacter winogradskyi grown in co-culture
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DOI：
10.1007/s00203-014-1056-1
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发表时间：
2015-01-01
影响因子：
2.8
通讯作者：
Sayavedra-Soto, Luis A.
中科院分区：
文献类型：
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作者：
Perez, Jose;Buchanan, Alex;Sayavedra-Soto, Luis A.
将欧洲亚硝酸单胞菌和葡萄酒硝化杆菌在恒温条件下单独培养和共培养，以探讨两种生长条件下的生理差异。含有60 mM NH4(+)的共培养基使细胞密度(0.20-0.29 OD600)大于单一恒化器培养的细胞密度之和，分别为0.09-0.14 OD600与60 mM NH4(+)，0.04-0.06 OD600(-)。依赖于NO2(-)和NH4(+)的O-2摄取率、qRT-PCR和显微镜观察表明，在共培养中，欧洲乳杆菌的摄氧率接近0.20OD600(接近80%)，而Winogradskyi的摄氧率接近0.05OD600(接近20%)。在共培养条件下，转录本结果表明，欧洲核桃中有773个基因(占基因总数的30.2%)和温氏核桃中的372个基因(占基因总数的11.8%)的基因表达水平发生了显著变化。总细胞生长和转录组分析表明，在共培养条件下，欧洲拟青霉的受益程度高于温格拉茨基拟青霉。
Nitrosomonas europaea and Nitrobacter winogradskyi were grown singly and in co-culture in chemostats to probe for physiological differences between the two growth conditions. Co-culture growth medium containing 60 mM NH4 (+) resulted in a cell density (0.20-0.29 OD600) greater than the sum of the densities in single chemostat cultures, i.e., 0.09-0.14 OD600 for N. europaea with 60 mM NH4 (+)and 0.04-0.06 OD600 for N. winogradskyi with 60 mM NO2 (-). The NO2 (-)- and NH4 (+)-dependent O-2 uptake rates, qRT-PCR, and microscopic observations indicated that in co-culture, N. europaea contributed similar to 0.20 OD600 (similar to 80 %) and N. winogradskyi similar to 0.05 OD600 (similar to 20 %). In co-culture, the transcriptomes showed that the mRNA levels of 773 genes in N. europaea (30.2 % of the genes) and of 372 genes in N. winogradskyi (11.8 % of the genes) changed significantly. Total cell growth and the analysis of the transcriptome revealed that in co-culture, N. europaea benefits more than N. winogradskyi.