A Mitochondria-Targeted Photosensitizer Showing Improved Photodynamic Therapy Effects Under Hypoxia
A Mitochondria-Targeted Photosensitizer Showing Improved Photodynamic Therapy Effects Under Hypoxia
复制标题
线粒体靶向光敏剂在缺氧条件下显示出改善的光动力治疗效果
DOI：
10.1002/anie.201604130
复制
发表时间：
2016-08-16
影响因子：
16.6
通讯作者：
Huang, Wei
中科院分区：
文献类型：
--
作者：
Lv, Wen;Zhang, Zhang;Huang, Wei
细胞器靶向光敏剂已被报道为有效的光动力治疗（PDT）药物。在这项工作中，我们设计并合成了两种铱（III）复合物，分别特异性地染色活细胞的线粒体和溶酶体。这两种配合物都表现出对氧猝灭敏感的长寿命磷光。在常氧和缺氧条件下评价了配合物的光细胞毒性。结果表明，线粒体靶向复合物处理的HeLa细胞维持较慢的呼吸速率，导致缺氧下细胞内氧水平升高。因此，与溶酶体靶向复合物相比，该复合物表现出更好的PDT效果，特别是在缺氧条件下，这表明线粒体靶向PDT药物在癌症治疗中具有更高的实用潜力。
Organelle-targeted photosensitizers have been reported to be effective photodynamic therapy (PDT) agents. In this work, we designed and synthesized two iridium(III) complexes that specifically stain the mitochondria and lysosomes of living cells, respectively. Both complexes exhibited long-lived phosphorescence, which is sensitive to oxygen quenching. The photocytotoxicity of the complexes was evaluated under normoxic and hypoxic conditions. The results showed that HeLa cells treated with the mitochondria-targeted complex maintained a slower respiration rate, leading to a higher intracellular oxygen level under hypoxia. As a result, this complex exhibited an improved PDT effect compared to the lysosome-targeted complex, especially under hypoxia conditions, suggestive of a higher practicable potential of mitochondria-targeted PDT agents in cancer therapy.