Coenzyme Q supplementation or over-expression of the yeast Coq8 putative kinase stabilizes multi-subunit Coq polypeptide complexes in yeast coq null mutants.
Coenzyme Q supplementation or over-expression of the yeast Coq8 putative kinase stabilizes multi-subunit Coq polypeptide complexes in yeast coq null mutants.
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DOI：
10.1016/j.bbalip.2013.12.017
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发表时间：
2014-04-04
期刊：
影响因子：
--
通讯作者：
Clarke CF
中科院分区：
文献类型：
--
作者：
He CH;Xie LX;Allan CM;Tran UC;Clarke CF
酵母菌中辅酶Q的生物合成需要一个多亚基辅酶Q多肽复合物。任何一个COQ基因的缺失都会导致呼吸缺陷和Coq4、Coq6、Coq7和Coq9多肽水平的降低，这表明它们在高分子质量复合物中的关联是稳定所必需的。在某些coq缺失突变体中，过度表达假定的Coq8激酶可以恢复敏感coq多肽的稳态水平，并促进后期q中间体的合成。通过二维blue-native/SDS PAGE对线粒体中地黄皂苷提取物的分离，我们发现在酵母coq零突变体中，Coq8的过表达深刻地影响了一些coq多肽在高分子质量复合物中的关联。Coq4多肽在coq3、coq5、coq6、coq7、coq9和coq10突变体中以高分子质量持续存在，表明Coq4是Coq复合物的中心组织者。补充外源Q6增加了Coq4、Coq7、Coq9和其他几种线粒体多肽的稳态水平，并促进了后期q中间体的形成。补充Q可以通过与一个或多个Coq多肽相互作用来稳定这个复合物。外源添加Q6或Coq8过表达的稳定作用取决于Coq1和Coq2产生聚异戊二烯中间体。基于观察到的Coq多肽的相互依赖性、外源Q6的影响以及对内源性聚异戊二烯中间体的需求，我们提出了一种新的q -生物合成复合物模型，称为Coq - synome。
Coenzyme Q biosynthesis in yeast requires a multi-subunit Coq polypeptide complex. Deletion of any one of the COQ genes leads to respiratory deficiency and decreased levels of the Coq4, Coq6, Coq7, and Coq9 polypeptides, suggesting that their association in a high molecular mass complex is required for stability. Over-expression of the putative Coq8 kinase in certain coq null mutants restores steady-state levels of the sensitive Coq polypeptides and promotes the synthesis of late-stage Q-intermediates. Here we show that over-expression of Coq8 in yeast coq null mutants profoundly affects the association of several of the Coq polypeptides in high molecular mass complexes, as assayed by separation of digitonin extracts of mitochondria by two-dimensional blue-native/SDS PAGE. The Coq4 polypeptide persists at high molecular mass with over-expression of Coq8 in coq3, coq5, coq6, coq7, coq9, and coq10 mutants, indicating that Coq4 is a central organizer of the Coq complex. Supplementation with exogenous Q6 increased the steady-state levels of Coq4, Coq7, Coq9, and several other mitochondrial polypeptides in select coq null mutants, and also promoted the formation of late-stage Q-intermediates. Q supplementation may stabilize this complex by interacting with one or more of the Coq polypeptides. The stabilizing effects of exogenously added Q6 or over-expression of Coq8 depend on Coq1 and Coq2 production of a polyisoprenyl intermediate. Based on the observed interdependence of the Coq polypeptides, the effect of exogenous Q6, and the requirement for an endogenously produced polyisoprenyl intermediate, we propose a new model for the Q-biosynthetic complex, termed the CoQ-synthome.
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DOI：
10.1083/jcb.93.1.97
发表时间：
1982-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.8
作者：
Do, TQ;Hsu, AY;Clarke, CF
通讯作者：
Clarke, CF
影响因子：
64.5
作者：
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通讯作者：
DOUGLAS, MG
DOI：
10.1111/j.1742-4658.2010.07862.x
发表时间：
2010-11
期刊：
The FEBS journal
影响因子：
--
作者：
Busso C;Tahara EB;Ogusucu R;Augusto O;Ferreira-Junior JR;Tzagoloff A;Kowaltowski AJ;Barros MH
通讯作者：
Barros MH