Dendrobine-type alkaloids and bibenzyl derivatives from Dendrobium findlayanum
Dendrobine-type alkaloids and bibenzyl derivatives from Dendrobium findlayanum
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石斛碱型生物碱和来自石斛兰的联苄衍生物
DOI：
10.1016/j.fitote.2020.104497
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发表时间：
2020-04-01
期刊：
影响因子：
3.4
通讯作者：
Xiao, Shi-Ji
中科院分区：
文献类型：
--
作者：
Liu, Gui-Yuan;Tan, Lu;Xiao, Shi-Ji
从兰花中分离得到5个化合物，其中包括2个石斛碱类生物碱（1和2），3个联苄基衍生物（3-5），沿着6个已知化合物。通过HR-ESIMS、NMR、旋光度和电子圆二色性（ECD）计算确定了化合物的结构和绝对构型。用MTT法测定化合物对A172、SHSY 5 Y和Hela三种人肿瘤细胞株的细胞毒作用。化合物6对三种人肿瘤细胞系显示出优异的抑制活性，IC 50范围为1.65 μ M至3.77 μ M。以尼罗红处理的斑马鱼为实验动物，研究了这些化合物对斑马鱼胃肠道运动的促进作用。化合物6在0.3 μ M的浓度下具有优异的促进斑马鱼胃肠运动的活性。
Five previously undescribed compounds, including two dendrobine-type alkaloids (1 and 2), three bibenzyl derivatives (3-5), along with six known compounds were isolated from orchids Dendrobium findlayanum. The structures and absolute configurations of the undescribed compounds were elucidated on the basis of HR-ESIMS, NMR spectroscopy, optical rotation value, as well as electronic circular dichroism (ECD) calculations. The cytotoxic effects of the isolated compounds on three human tumour cell lines (A172, SHSY5Y, and Hela) were evaluated by the MTT assay. Compound 6 showed excellent inhibitory activities against three human tumour cell lines with IC50 ranging from 1.65 mu M to 3.77 mu M. All these compounds were assessed for their activity of promoting the gastrointestinal motility of zebrafish treated with Nile red. Compound 6 have excellent activity to promote the gastrointestinal motility of zebrafish at the concentration of 0.3 mu M.