Preparation of water-soluble chitosan/heparin complex and its application as wound healing accelerator
Preparation of water-soluble chitosan/heparin complex and its application as wound healing accelerator
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DOI：
10.1016/s0142-9612(02)00566-5
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发表时间：
2003-04-01
期刊：
影响因子：
14
通讯作者：
Park, YY
中科院分区：
文献类型：
--
作者：
Kweon, DK;Song, SB;Park, YY
为了制备有效的伤口愈合促进剂，利用具有伤口愈合能力的水溶性壳聚糖（WSC）和具有吸引或结合与伤口愈合过程相关的生长因子能力的肝素（CH）制备了水溶性壳聚糖/肝素复合物。将WSC与肝素反应制备水溶性CH复合物，再加入蒸馏水制成高粘度软膏剂。为了评价伤口愈合效果，在大鼠背部进行全层皮肤切除，然后分别在伤口中施用WSC和水溶性CH复合物软膏。15天后，进行大体和组织学检查。大体上，未处理对照组显示伤口边缘清晰，并被结痂覆盖。用WSC软膏治疗的第二组显示伤口尺寸小，覆盖痂量少，边缘不清，似乎从边缘再生。用水溶性CH复合物软膏治疗的第三组似乎几乎完全愈合。各组的组织学与大体结果具有良好的相关性。与对照组和第二组相比，第三组显示出与真皮中的正常相似的附件结构的几乎完全再生，第二组分别没有皮肤附件和皮肤附件数量较少。(C)2002爱思唯尔科技有限公司版权所有。
To make effective wound healing accelerator, water-soluble chitosan (WSC)/heparin (CH) complex was prepared using WSC with wound healing ability and heparin with ability to attract or bind growth factor related to wound healing process. Water-soluble CH complex was prepared by the reaction between WSC and heparin, and then, by adding distilled water to it, ointment type with high viscosity was made. To evaluate the wound healing effect, full thickness skin excision was performed on the backs of the rat and then WSC and water-soluble CH complex ointments were applied in the wounds, respectively. After 15 days, gross and histologic examination was performed. Grossly, untreated control group revealed that the wound had well defined margin and was covered by crust. The second group treated with WSC ointment revealed small wound size with less amount of covering crust and ill-defined margin, which appeared to regenerate from margin. The third group treated with water-soluble CH complex ointment appeared to be nearly completely healed. Histology of each group was well correlated to gross findings. The third group shows nearly complete regeneration of appendage structure similar to normal in the dermis in contrast to control and second group with absence and less number of skin appendages, respectively. (C) 2002 Elsevier Science Ltd. All rights reserved.