THE APPLICATION OF A CAPACITIVE PROBE TECHNIQUE FOR DIRECT OBSERVATION OF ELECTROMECHANICAL PROCESSES IN THE GUINEA-PIG COCHLEA
THE APPLICATION OF A CAPACITIVE PROBE TECHNIQUE FOR DIRECT OBSERVATION OF ELECTROMECHANICAL PROCESSES IN THE GUINEA-PIG COCHLEA
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DOI：
10.1121/1.395556
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发表时间：
1987-07-01
影响因子：
2.4
通讯作者：
LEPAGE, EL
中科院分区：
文献类型：
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作者：
LEPAGE, EL
哺乳动物耳蜗外毛细胞诱发力学反应的间接测量为直接力学测量带来了一系列新的问题。直接和间接测量表明，诱发的机电反应的频谱可以很好地扩展到音频范围，这表明需要制定术语和协议来区分诱发的机械反应和传统的行波，当两者明显地叠加在正常功能的耳蜗基底膜的运动上时。详细介绍了一种调频电容式探针技术，用于测量豚鼠耳的振动结构。考虑因素包括换能器的设计、校准、灵敏度、线性度和噪声源，以及该技术对动物准备的影响，特别是与测量中排空鼓室相关的问题。将该技术的相对优缺点与目前可用的其他技术的显著特征进行了比较。鉴于耳蜗力学的明显复杂性，需要一些初步的实验来阐明反转导过程的一些关键问题。一个“简单”的第一个实验是测试是否存在任何纠正或运动反应。
The demonstration of evoked mechanical responses of the outer hair cells in the mammalian cochlea by indirect measurements introduces a new range of problems into direct mechanical measurements. Direct and indirect measurements indicate that the frequency spectra of evoked electromechanical responses may extend well into the range of audio frequencies, revealing a need to develop terminology and protocols for distinguishing evoked mechanical responses from the traditional traveling wave when both are apparently superimposed on the motion of the basilar membrane in the normally functioning cochlea. Details are presented of a frequency-modulation capacitive probe technique for measurement of vibrating structures of the guinea pig ear. Consideration include the design of the transducer, calibration, sensitivity, linearity, and sources of noise, as well as the influence of the technique upon the animal preparation, and in particular the issues associated with draining scala tympani for the measurement. Relative advantages and disadvantages of the technique are compared with salient features of other techniques currently available. In view of the apparent complexity of cochlear mechanics some preliminary experiments are required to elucidate some of the key questions about reverse-transduction processes in general. A "simple" first experiment is to test existence of any rectifying or motile response.