Rh glycoprotein immunoreactivity in the skin and its role in extrabranchial ammonia excretion by the sea lamprey ( Petromyzon marinus ) in fresh water
Rh glycoprotein immunoreactivity in the skin and its role in extrabranchial ammonia excretion by the sea lamprey ( Petromyzon marinus ) in fresh water
复制标题
皮肤中的Rh糖蛋白免疫反应性及其在淡水中七鳃鳗（Petromyzon marinus）鳃外氨排泄中的作用
DOI：
10.1139/cjz-2016-0120
复制
发表时间：
2017
影响因子：
1.4
通讯作者：
Edwards, S.L.
中科院分区：
文献类型：
--
作者：
Blair, S.D.;Wilkie, M.P.;Edwards, S.L.
水生生物采用各种策略通过鳃、皮肤和（或）肾途径排泄氨。在它们生命周期的三个不同阶段，我们假设基底脊椎动物海七鳃鳗（Petromyzon marinusL.，1758）使用皮肤作为氨排泄的途径。氨排泄量的测量，使用划分通量室发现，鳃外的网站（皮肤加肾）的氨排泄量更重要的是在幼虫海七鳃鳗，但变态后，鳃成为主要的排泄途径在少年海七鳃鳗。尽管更大的相对重要性，在幼虫阶段的皮肤，Rh糖蛋白亚型Rhbg，Rhcg 1，和Rhcg 2在皮肤中检测到在所有三个海七鳃鳗生命阶段检查，但表达的模式依赖于生命阶段。我们的结论是，在相对久坐不动的滤食性幼虫阶段，鳃外途径发挥同样重要的作用，鳃在促进氨排泄。然而，鳃凭借其广泛的鳃脉管系统成为变态后氨排泄的主要途径，因为需要卸载更大量的氨，这是由更高的基础氨产生率和在寄生阶段摄入富含蛋白质的血液后排泄更大量的氨的潜力引起的。
Aquatic organisms employ various strategies to excrete ammonia across the gills, skin, and (or) renal routes. During three different stages of their life cycle, we hypothesized that the basal vertebrate sea lamprey (Petromyzon marinusL., 1758) used the skin as a route for ammonia excretion. Measurements of ammonia excretion using divided flux chambers revealed that extrabranchial sites (skin plus renal) of ammonia excretion were quantitatively more important in larval sea lampreys, but following metamorphosis, the gills became the dominant route of excretion in juvenile sea lampreys. Despite the greater relative importance of the skin in the larval stage, Rh glycoprotein isoforms Rhbg, Rhcg1, and Rhcg2 were detected in the skin in all three sea lamprey life stages examined, but the patterns of expression were dependent on the life stage. We conclude that, during the relatively sedentary filter-feeding larval stage, extrabranchial routes play an equally important role as the gill in facilitating ammonia excretion. However, the gills by virtue of their extensive branchial vasculature become the dominant route of ammonia excretion following metamorphosis because of the need to offload greater amounts of ammonia arising from higher rates of basal ammonia production and the potential to excrete higher amounts of ammonia following ingestion of protein-rich blood in the parasitic stage.