Effect of glutamine dipeptide on hepatic regeneration in partially hepatectomized malnourished rats
Effect of glutamine dipeptide on hepatic regeneration in partially hepatectomized malnourished rats
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DOI：
10.1016/s0899-9007(03)00177-1
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发表时间：
2003-11-01
期刊：
影响因子：
4.4
通讯作者：
Waitzberg, DL
中科院分区：
文献类型：
--
作者：
Mazza, RPD;Bertevello, PL;Waitzberg, DL
目的：谷氨酰胺促进营养（N）大鼠的肝再生。本研究的目的是评估富含谷氨酰胺的全肠外营养 (TPN) 对营养不良 (MN) 大鼠肝再生的影响。 方法：72 只雄性 Wistar 大鼠（相当于 270 克）被分配到两组中的一组：N 组和 MN 组。然后将每组再分为三组：第一组接受部分肝切除术（PH）并接受富含L-丙氨酰-L-谷氨酰胺（Ala-Gln）的标准TPN；第二个也进行了 PH 并接受了标准 TPN，但添加了含有脯氨酸和丙氨酸 (Ala-Pro) 的溶液；第三组没有接受手术（对照组）。所有实验组均接受等热量（188 kcal (.) kg(-1) (.) d(-1)）和等氮（1.12 g 氮 (.) kg(-1) (.) d(-1)）TPN 96 小时。所有动物在死亡前2小时注射溴脱氧尿苷。分析肝再生指数（HRI）、肝生长百分比（HG）和肝脏形态。结果：在 MN 大鼠中，谷氨酰胺富集组（HRI = 81 和 HG = 190）的 HRI 和 HG 高于含脯氨酸的 TPN（HRI = 66 和 HG = 154；P < 0.05），并且接受 PH 的动物的 HRI 比对照组高 100 倍。动物。六组肝组织形态学分析显示无差异。结论：富含谷氨酰胺的TPN促进了PH后MN大鼠残肝的生长，维持了术后各实验组的细胞增殖，并维持了MN大鼠术后的肝脏形态。 (C) 爱思唯尔公司，2003 年。
OBJECTIVE: Glutamine promotes hepatic regeneration in nourished (N) rats. The aim of the present study was to evaluate the effects of glutamine-enriched total parenteral nutrition (TPN) on liver regeneration in malnourished (MN) rats.METHODS: Seventy-two male Wistar rats (congruent to270 g) were assigned to one of two groups: N and MN. Each group was then subdivided into three groups: the first underwent partial hepatectomy (PH) and received standard TPN enriched with L-alanyl-L-glutamine (Ala-Gln); the second also underwent PH and received standard TPN, but enriched with a solution containing proline and alanine (Ala-Pro); and the third underwent no surgical procedure (control group). All experimental groups received isocaloric (188 kcal (.) kg(-1) (.) d(-1)) and isonitrogenous (1.12 g of nitrogen (.) kg(-1) (.) d(-1)) TPN for 96 h. All animals were injected with bromodeoxyuridine 2 h before death. The hepatic regeneration index (HRI), hepatic growth percentage (HG) and hepatic morphology were analyzed.RESULTS: In MN rats, HRI and HG were higher with glutamine enrichment (HRI = 81 and HG = 190) than with proline-containing TPN (HRI = 66 and HG = 154; P < 0.05) and HRI was 100 times higher in animals that underwent PH than in control animals. Morphologic analysis of hepatic tissue showed no difference among the six groups.CONCLUSION: Glutamine-enriched TPN promoted growth of the remnant liver in MN rats after PH, maintained cellular proliferation in the various experimental groups after surgery, and maintained hepatic morphology of MN rats after surgery. (C) Elsevier Inc. 2003.