Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry
Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry
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DOI：
10.1016/s0378-1135(00)00197-8
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发表时间：
2000-06-12
影响因子：
3.3
通讯作者：
Jensen, LB
中科院分区：
文献类型：
--
作者：
Aarestrup, FM;Agerso, Y;Jensen, LB
对丹麦禽类感染的118株葡萄球菌的种类分布、对19种抗菌药的敏感性以及对大环内酯类、链球菌和四环素类抗生素的耐药基因进行了检测。使用常规生化测试和16S rDNA测序相结合的方法对分离株进行鉴定。最常见的是金黄色葡萄球菌(83株)、海氏葡萄球菌(11株)、木质葡萄球菌(9株)和科氏葡萄球菌(6株)。这些分离株对大多数测试的抗菌剂都敏感。金黄色葡萄球菌对环丙沙星耐药率较高(30%)。只有6株(7%)金黄色葡萄球菌和1株腐生葡萄球菌对青霉素耐药。16株(19%)金黄色葡萄球菌和2株凝固酶阴性葡萄球菌(CNS)对磺胺甲恶唑耐药。20株(24%)金黄色葡萄球菌对红霉素耐药，其中19株含有ermA基因，其余1株含有ermC基因。11株(48%)对新诺明耐药的中枢神经系统对红霉素耐药，且全部携带ermA基因。鉴定为木糖链球菌的两个分离物对链球菌具有抗性，并且都含有vatB-和vgaB-基因。39株(47%)金黄色葡萄球菌、9株海链球菌中的3株和23株新霉素耐药的CNS中的8株对四环素耐药，均含有TCT(K)基因。一株金黄色葡萄球菌分离株也含有tet(M)基因。本研究表明，丹麦肉鸡葡萄球菌对氟喹诺酮类、四环素类和大环内酯类药物的耐药率较高，而对其他抗菌药物的耐药率较低。检测到了与先前观察到的编码红霉素、四环素和链球菌耐药性的基因相似的基因。(C)2000 Elsevier Science B.V.保留所有权利。
The species distribution, susceptibility to 19 antimicrobial agents and presence of selected genes encoding resistance to macrolides, streptogramins and tetracyclines were examined among 118 staphylococcal isolates from infections of poultry in Denmark. Isolates were identified using a combination of conventional biochemical testing and 16S rDNA sequencing. The most common species were Staphylococcus aureus (83), Staphylococcus hyicus (11), Staphylococcus xylosus (9) and Staphylococcus cohnii (6). The isolates were susceptible to most antimicrobials tested. A high frequency of S. aureus (30%) was resistant to ciprofloxacin. Only six (7%) S. aureus isolates and one Staphylococcus saprophyticus were penicillin resistant. Resistance to sulphamethoxazole was observed among 16 (19%) of S. aureus isolates and two coagulase negative staphylococci (CNS). Twenty (24%) of the S. aureus isolates were resistant to erythromycin and 19 of these isolates contained the ermA gene, whereas the remaining isolate contained the ermC gene. Eleven (48%) of the novobiocin resistant CNS were resistant to erythromycin and all these isolates contained the ermA gene. Two isolates identified as S. xylosus, were found to be resistant to streptogramins and both contained the vatB- and the vgaB-genes. Thirty-nine (47%) of the S. aureus isolates, three of nine S. hyicus and eight of the 23 novobiocin resistant CNS were tetracycline resistant and all contained the tct(K) gene. A single S. aureus isolate also contained the tet(M) gene. The present study showed a frequent occurrence of resistance to fluoroquinolones, tetracycline and macrolides among staphylococci isolated from broilers in Denmark, whereas the occurrence of resistance to other antimicrobial agents remains low. Similar genes, encoding resistance to erythromycin, tetracycline and streptogramins to those previously observed, were detected. (C) 2000 Elsevier Science B.V. All rights reserved.