Immunotopographical Differences of Human Skin.
Immunotopographical Differences of Human Skin.
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DOI：
10.3389/fimmu.2018.00424
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发表时间：
2018
影响因子：
7.3
通讯作者：
Szegedi A
中科院分区：
文献类型：
--
作者：
Béke G;Dajnoki Z;Kapitány A;Gáspár K;Medgyesi B;Póliska S;Hendrik Z;Péter Z;Törőcsik D;Bíró T;Szegedi A
健康皮肤的免疫屏障被认为在整个身体表面上是统一的-然而，最近的间接发现挑战了这一教条，因为微生物和化学环境（例如，皮脂、汗液和pH）在地形上不同的皮肤区域上表现出显著的差异。因此，在本研究中，我们进行了全转录组和后续途径分析，以评估皮脂腺丰富（SGR）和皮脂腺贫乏（SGP）区域之间的差异。在这里，我们提供了第一个证据，表明不同的皮肤区域表现出特征性的先天性和适应性免疫和屏障环境，因为我们可以检测到显着增加的趋化因子。（CCL 2，3，19，20，23，24）和抗微生物肽（S100 A7、A8、A9、脂质运载蛋白、β-防御素-2）表达、改变的屏障（角蛋白17、79）功能以及与SGP相比SGR皮肤中的非炎性Th 17/IL-17优势。关于促炎分子（IL-1α、IL-6、IL-8、IL-33、TNF-α），在两个区域均检测到相似的低水平。我们的数据可以解释一些免疫介导的和自身免疫性皮肤病的特征性局部定位，我们还建议，在实验皮肤病学中广泛使用的术语“健康皮肤对照样本”，只有在研究人员仔细指定健康皮肤的确切区域（沿着患病样本的部位）时才应该被接受。
The immunological barrier of the healthy skin is considered to be unified on the whole body surface—however, recent indirect findings have challenged this dogma since microbial and chemical milieu (e.g., sebum, sweat, and pH) exhibit remarkable differences on topographically distinct skin areas. Therefore, in the present study, we performed whole transcriptomic and subsequent pathway analyses to assess differences between sebaceous gland rich (SGR) and sebaceous gland poor (SGP) regions. Here, we provide the first evidence that different skin regions exhibit a characteristic innate and adaptive immune and barrier milieu as we could detect significantly increased chemokine (CCL2, 3, 19, 20, 23, 24) and antimicrobial peptide (S100A7, A8, A9, lipocalin, β-defensin-2) expression, altered barrier (keratin 17, 79) functions, and a non-inflammatory Th17/IL-17 dominance in SGR skin compared to SGP. Regarding pro-inflammatory molecules (IL-1α, IL-6, IL-8, IL-33, TNF-α), similarly low levels were detected in both regions. Our data may explain the characteristic topographical localization of some immune-mediated and autoimmune skin disorders and we also propose that the term “healthy skin control sample,” widely used in experimental Dermatology, should only be accepted if researchers carefully specify the exact region of the healthy skin (along with the site of the diseased sample).