Overexpression of a chrysanthemum transcription factor gene DgNAC1 improves the salinity tolerance in chrysanthemum
Overexpression of a chrysanthemum transcription factor gene DgNAC1 improves the salinity tolerance in chrysanthemum
复制标题
DOI：
10.1007/s00299-017-2103-6
复制
发表时间：
2017-04-01
影响因子：
6.2
通讯作者：
Liu, Guang-li
中科院分区：
文献类型：
--
作者：
Wang, Ke;Zhong, Ming;Liu, Guang-li
菊花转录因子DgNAC1在功能上是通过调节胁迫响应基因来实现对盐胁迫的响应，NAC转录因子在不同的非生物胁迫抗性中起着有效的作用，NAC转录因子在拟南芥中的过表达被证明有助于提高耐盐性。然而，菊花中NAC基因的功能仍然知之甚少。在这里，我们进行了生理学和分子实验，以评估DgNAC1在菊花盐胁迫反应中的作用。在本研究中，过表达DgNAC1的菊花明显比野生型(WT)更耐盐。转基因菊花在盐胁迫下表现出比对照菊花更高的存活率和更低的电导率。在盐胁迫下，转基因菊花体内丙二醛(MDA)和活性氧(H_2O_2、O_2~(-2))的积累减少，超氧化物歧化酶(SOD)、过氧化氢酶(POD)和过氧化氢酶(CAT)活性增加，脯氨酸含量增加。此外，在盐分胁迫下，转基因菊花的胁迫响应基因表达上调幅度大于野生型菊花。因此，DgNAC1基因可能是耐盐植物分子育种中的一个重要基因。
DgNAC1, a transcription factor of chrysanthemum, was functionally verified to confer salt stress responses by regulating stress-responsive genes.NAC transcription factors play effective roles in resistance to different abiotic stresses, and overexpressions of NAC TFs in Arabidopsis have been proved to be conducive in improving salinity tolerance. However, functions of NAC genes in chrysanthemum continue to be poorly understood. Here, we performed physiology and molecular experiments to evaluate roles of DgNAC1 in chrysanthemum salt stress responses. In this study, DgNAC1-overexpressed chrysanthemum was obviously more resistant to salt over the WT (wild type). Specifically, the transgenic chrysanthemum showed a higher survival rate and lower EC (electrolyte conductivity) than WT under salt stress. The transgenic chrysanthemum also showed fewer accumulations of MDA (malondialdehyde) and reactive oxygen species (H2O2 and O-2 (-)), greater activities of SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase), as well as more proline content than WT under salt stress. Furthermore, stress-responsive genes in transgenic chrysanthemum were greater up-regulated than in WT under salinity stress. Thus, all results revealed that DgNAC1 worked as a positive regulator in responses to salt stress and it may be an essential gene for molecular breeding of salt-tolerant plants.