Quantification of Total Phenolics, Flavonoids and Evaluation of in vitro Free Radical Scavenging Activities in Psidium guajava L.
Quantification of Total Phenolics, Flavonoids and Evaluation of in vitro Free Radical Scavenging Activities in Psidium guajava L.
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DOI：
10.36468/pharmaceutical-sciences.683
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发表时间：
2020-07-01
影响因子：
0.5
通讯作者：
Anandhi, D. Usha
中科院分区：
文献类型：
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作者：
Sowmya, B. H.;Anandhi, D. Usha
本研究旨在量化总酚类和黄酮类化合物，并研究两种番石榴品种 Lalit 和 Allahabad safeda 叶提取物的体外自由基清除活性。通过索氏提取法制备 Lalit 和 Allahabad safeda 的叶提取物，并使用乙醇提取物进行体外测定。测定 Lalit 和 Allahabad 浓度为 100、200、300、400、500、600 和 700 μg/ml 时的总酚和类黄酮含量、2,2-偶氮双(3-乙基苯并噻唑啉-6-磺酸)、2,2-二苯基-1-苦基肼、一氧化氮和超氧化物自由基清除活性萨费达叶 提取物。结果表明，与 Allahabad safeda 相比，Lalit 叶提取物中的酚类和类黄酮含量更高。 Lalit 和 Allahabad safeda 的叶提取物表现出剂量依赖性的 2,2-azino-bis(3-乙基苯并噻唑啉-6-磺酸)、2,2-二苯基-1-三硝基苯肼和一氧化氮自由基清除活性的增加以及超氧自由基清除活性的降低。然而，与 Allahabad safeda 相比，Lalit 显示出更高的自由基清除活性。此外，番石榴对DPPH、2,2-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)、一氧化氮和超氧化物自由基的IC50值分别为229.56、503.07、430.40、07和377.96μg/ml。此外，Allahabad safeda对2,2-二苯基-1-三硝基苯肼、2,2-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)、一氧化氮和超氧化物自由基的IC50值分别为270.24、543.63、469.99和515.50μg/ml。这项研究清楚地证明了拉利特和阿拉哈巴德安全提取物的抗氧化活性。与 Allahabad safeda 相比，Lalit 提取物具有更好的抗氧化活性，可能是因为其酚类含量更高。
The present study was undertaken to quantify total phenolics and flavonoids and investigate in vitro free radical scavenging activities of leaf extracts of two varieties of Psidium guajava L. viz., Lalit and Allahabad safeda. Leaf extracts of Lalit and Allahabad safeda were prepared by Soxhlet extraction and ethanol extracts were used for in vitro assays. Total phenolic and flavonoid contents, 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, nitric oxide and super oxide free radical scavenging activities were determined at 100, 200, 300, 400, 500, 600 and 700 mu g/ml concentrations of Lalit and Allahabad safeda leaf extracts. Results indicated the presence of higher phenolic and flavonoid contents in the leaf extract of Lalit compared to that of Allahabad safeda. The leaf extracts of Lalit and Allahabad safeda showed dose-dependent increase in 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl and nitric oxide radical scavenging activities and a decrease in super oxide radical scavenging activities. However, Lalit showed higher free radical scavenging activity compared to that of Allahabad safeda. Further, the IC50 values of Psidium guajava for DPPH, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), nitric oxide and super oxide free radicals were 229.56, 503.07, 430.40, 07 and 377.96 mu g/ml, respectively. In addition, the IC50 values of Allahabad safeda for 2,2-diphenyl-1-picrylhydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), nitric oxide and super oxide free radicals were, 270.24, 543.63, 469.99 and 515.50 mu g/ml, respectively. This study clearly demonstrated antioxidant activity in both the extracts of Lalit and Allahabad safeda. The better antioxidant activity of Lalit extract could be due to the presence of higher phenolic content compared to Allahabad safeda.