Transcriptomic diversification of granulosa cells during follicular development between White Leghorn and Silky Fowl hens.
Transcriptomic diversification of granulosa cells during follicular development between White Leghorn and Silky Fowl hens.
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白来亨鸡和乌骨鸡卵泡发育过程中颗粒细胞的转录组多样性
DOI：
10.3389/fgene.2022.965414
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发表时间：
2022
影响因子：
3.7
通讯作者：
Deng, Xuemei
中科院分区：
文献类型：
--
作者：
Tai, Yurong;Yang, Xue;Han, Deping;Xu, Zihan;Cai, Ganxian;Hao, Jiaqi;Zhang, Bingjie;Deng, Xuemei
鸡的产蛋率与卵泡发育的连续性有关。在本研究中，我们发现高产蛋鸡品种白来航鸡(WL)的白色、显性和黄色排卵前卵泡数显著高于低产蛋鸡品种(SF)。颗粒细胞(GCs)的增殖和分化在卵泡成熟过程中起重要作用。组织学观察发现，SF组卵泡外颗粒层有大量的黑素细胞，而WL组未见。最后，利用RNA测序技术分析了WL母鸡和SF母鸡卵泡中GC层的基因表达谱和途径。对WL和SF之间的层序前GCs(PhGCs)和排卵前GCs(PoGCs)的转录组分析表明，与类固醇激素、催产素合成、紧密连接和内吞相关的基因在WL phGCs中的表达水平高于SF phGCs，而胰岛素信号通路和血管平滑肌收缩相关基因在SF phGCs中表达上调。脂肪酸合成、钙信号和Wnt信号通路相关基因在WL poGCs中的表达水平高于SF poGCs，而肾上腺素能信号、cGMP-PKG和黑素生成相关基因在SF poGCs中表达上调。这些结果表明，促进GC增殖和各种性激素分泌的基因在WL母鸡中比在SF母鸡中更活跃。SF中上调的信号通路有助于为GC以及血管生成和黑素生成提供能量。体外实验证实，WL母鸡的poGCs增殖和生殖激素合成均高于SF母鸡。
Egg production rate in chicken is related to the continuity of follicle development. In this study, we found that the numbers of white prehierarchical, dominant, and yellow preovulatory follicles in the high-yielding layer breed, White Leghorn (WL), were significantly higher than those in the low egg-yielding variety, Silky Fowl (SF). The proliferation and differentiation of granulosa cells (GCs) play an important role in follicle maturation. Histological observation revealed a large number of melanocytes in the outer granulosa layer of follicles in SF but not in WL. Finally, RNA-sequencing was used to analyze the gene expression profiles and pathways of the GC layer in the follicles in both WL and SF hens. Transcriptome analysis of prehierarchical GCs (phGCs) and preovulatory GCs (poGCs) between WL and SF showed that steroid hormone-, oxytocin synthesis-, tight junction-, and endocytosis-related genes were expressed at higher levels in WL phGCs than in SF phGCs, whereas the insulin signaling pathway- and vascular smooth muscle contraction-related genes were upregulated in SF phGCs. Fatty acid synthesis, calcium signaling, and Wnt signaling pathway-related genes were expressed at higher levels in WL poGCs than in SF poGCs; however, adrenergic signaling, cGMP-PKG, and melanogenesis-related genes were upregulated in SF poGCs. These results indicate that genes that promote GC proliferation and secretion of various sex hormones are more active in WL than in SF hens. The upregulated signaling pathways in SF help in providing energy to GCs and for angiogenesis and melanogenesis. In vitro experiments confirmed that both the proliferation of poGCs and synthesis of reproductive hormones were higher in WL than in SF hens.
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影响因子：
14.9
作者：
Lopez-Garcia J;Periyasamy M;Thomas RS;Christian M;Leao M;Jat P;Kindle KB;Heery DM;Parker MG;Buluwela L;Kamalati T;Ali S
通讯作者：
Ali S
影响因子：
3.7
作者：
Brady K;Liu HC;Hicks JA;Long JA;Porter TE
通讯作者：
Porter TE
影响因子：
4.1
作者：
Braw-Tal, R
通讯作者：
Braw-Tal, R
影响因子：
2.8
作者：
Davoodi, S.;Cooke, R. F.;Cerri, R. L. A.
通讯作者：
Cerri, R. L. A.
影响因子：
1.7
作者：
Evans, ACO
通讯作者：
Evans, ACO