Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries
Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries
复制标题
链球菌抗原I/II单克隆抗体局部被动免疫预防龋齿
作者：
T. Lehner;J. Caldwell;R. Smith
用变形链球菌单抗(Mc-Ab)局部被动免疫是主动系统免疫的一种替代方法。我们制备了一种针对链球菌抗原I/II细胞表面蛋白决定簇的G类Mc抗体，并将其反复应用于恒河猴的牙齿。这导致变形链球菌在牙齿缝隙和光滑表面的定植减少，并且没有龋齿，与对照组动物的结果不同，对照组动物患龋齿，牙齿上显示高比例的变形链球菌。在两组动物之间，血清、唾液或牙周液中对变形链球菌的抗体没有显著差异。因此，任何对诱导交叉反应抗体的系统免疫提出的反对意见都可以通过局部被动免疫来克服。其防止定植的机制尚未建立，但我们推测，针对变形链球菌的一个重要细胞表面抗原决定簇(链球菌抗原I/II)的Mc抗体可以阻止变形链球菌附着在牙齿表面获得性膜上。变形链球菌与Mc抗体发生反应，并被嗜中性粒细胞的局部牙周运输调理、吞噬和杀死。
Local passive immunization with monoclonal antibodies (Mc Ab) to Streptococcus mutans was attempted as an alternative approach to active systemic immunization. We prepared an immunoglobulin G class Mc Ab to the cell surface protein determinant of streptococcal antigen I/II and applied it repeatedly to the teeth of rhesus monkeys. This resulted in decreased colonization by S. mutans in fissures and smooth surfaces of teeth and no dental caries, unlike the results in control animals, which developed caries and showed a high proportion of S. mutans on their teeth. There was no significant difference in serum, salivary, or gingival fluid antibodies to S. mutans between the two groups of animals. Any objections raised over systemic immunization inducing cross-reactive antibodies are therefore overcome by local passive immunization. The mechanism of prevention of colonization has not been established, but we postulate that the Mc Ab which is directed against an important cell surface antigenic determinant of S. mutans (streptococcal antigen I/II) prevents adherence of S. mutans to the acquired pellicle on the tooth surface. S. mutans reacts with the Mc Ab and becomes opsonized, phagocytosed, and killed by the local gingival traffic of neutrophils.