SYNTHESIS, STORAGE AND RELEASE OF ACETYLCHOLINE BY A NORADRENERGIC PHEOCHROMOCYTOMA CELL LINE
SYNTHESIS, STORAGE AND RELEASE OF ACETYLCHOLINE BY A NORADRENERGIC PHEOCHROMOCYTOMA CELL LINE
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DOI：
10.1038/268349a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
REIN, G
中科院分区：
文献类型：
--
作者：
GREENE, LA;REIN, G
发育中的神经嵴组织在神经递质合成方面表现出一定程度的可塑性。例如，假定的去甲肾上腺素能组织的异位移植可能会产生胆碱能特性，反之亦然1,2。此外，合成儿茶酚胺的大鼠交感神经元的单层培养物可能通过暴露于神经节非神经元细胞或由此类细胞调节的培养基而影响乙酰胆碱的合成3,4。最近，从可移植的大鼠肾上腺髓质嗜铬细胞瘤6中建立了克隆细胞系（命名为PC12）。该细胞系表达了神经嵴来源的去甲肾上腺嗜铬细胞和交感神经元的许多特征，包括儿茶酚胺的合成、储存、摄取和特异性释放5,7,8。此外，在接触神经生长因子（或 NGF，一种影响正常交感神经元生长和发育的蛋白质 9）后的几天内，PC12 细胞停止有丝分裂活动并延长较长的神经元过程 5。我们在此表明，PC12 细胞的去甲肾上腺素能培养物也可以合成、储存和释放乙酰胆碱。
DEVELOPING neural crest tissues show a certain degree of plasticity with respect to neurotransmitter synthesis. For example, heterotopic transplants of presumptive noradrenergic tissue may develop cholinergic properties and vice versa1,2. Also, monolayer cultures of rat sympathetic neurones which synthesise catecholamines may be influenced to synthesise acetylcholine by exposure to ganglionic non-neuronal cells or to medium conditioned by such cells3,4. Recently, a clonal cell line (designated PC12) was established5from a transplantable rat adrenal medullary pheochromocytoma6. This line expresses many characteristic properties of neural-crest-derived noradernergic adrenal chromaffin cells and sympathetic neurones, including synthesis, storage, uptake and specific release of catecholamines5,7,8. In addition, within several days after their exposure to nerve growth factor (or NGF, a protein which influences the growth and development of normal sympathetic neurones9), PC12 cells cease mitotic activity and extend long, neuronal processes5. We show here that noradrenergic cultures of PC12 cells can also synthesise, store and release acetylcholine.