The structure of the cuticular plate, an in vivo actin gel.
The structure of the cuticular plate, an in vivo actin gel.
复制标题
表皮板的结构，一种体内肌动蛋白凝胶。
DOI：
10.1083/jcb.109.6.2853
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发表时间：
1989-12
影响因子：
7.8
通讯作者：
Tilney, L G
中科院分区：
文献类型：
--
作者：
DeRosier, D J;Tilney, L G
角质板是在耳蜗毛细胞中发现的肌动蛋白丝网络。在鳄鱼蜥蜴中，它由从静纤毛发出的小根和交叉连接的肌动蛋白丝组成，这些肌动蛋白丝锚定这些小根。在薄切片中，这个网络显示出650 +/- 110-A的条纹的醒目补丁。通过对图像的定量分析，可以解释条纹的奥秘。它们部分是由于小根是一组肌动蛋白丝的扁平带状物。每组中的带被分开约650 A。许多直径为30 A的晶须从每个带的表面延伸，使相邻的带相互连接。除细胞边缘外，无根的花丝主要以单丝形式出现。像丝带一样，它们也长满了胡须。条纹的斑块是由从它们突出的胡须以650埃的间隔保持的带状和丝状物解释的。有胡须的细丝区域的一个简单模型是一个装满随机取向的试管刷的盒子。通过盒子的薄片将显示由于刷的钢丝骨架通过刷毛长度彼此分开而导致的暗线或条纹区域。用计算机代替试管刷，我们已经能够定量地模拟蜥蜴表皮板上可见的细丝分布和条纹图案。我们从模拟中推断出的肌动蛋白丝的组织与巨噬细胞或肠上皮细胞末端网中发现的不同。
The cuticular plate is a network of actin filaments found in hair cells of the cochlea. In the alligator lizard, it consists of rootlets, emanating from the stereocilia, and of cross-connecting actin filaments that anchor these rootlets. In thin sections, this network displays striking patches of 650 +/- 110-A striae. By quantitative analyses of the images, the mystery of the striae can be explained. They are due in part to the rootlets which are sets of flat ribbons of actin filaments. The ribbons in each set are separated by approximately 650 A. Numerous whiskers 30 A in diameter extend from each ribbon's face, interconnecting adjacent ribbons. The nonrootlet filaments, except at the margins of the cell, occur primarily as single filaments. Like the ribbons, they are bristling with whiskers. The patches of striae are explained by ribbons and filaments held at a 650-A separation by the whiskers that project from them. A simple model for regions of bewhiskered filaments is a box crammed full of randomly oriented test- tube brushes. A thin slice through the box will show regions of dark lines or striae due to the wire backbones of the brushes separated from one another by the bristle length. Using the computer instead of test- tube brushes, we have been able to model quantitatively the filament distribution and pattern of striae seen in the cuticular plate of the lizard. The organization of actin filaments we have deduced from our simulations differs from that found in macrophages or in the terminal web of intestinal epithelial cells.