Electroosmotic properties of microfluidic channels composed of poly(dimethylsiloxane)
Electroosmotic properties of microfluidic channels composed of poly(dimethylsiloxane)
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DOI：
10.1016/s0378-4347(01)00327-9
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发表时间：
2001-10-25
期刊：
影响因子：
--
通讯作者：
Allbritton, N
中科院分区：
文献类型：
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作者：
Ren, XQ;Bachman, M;Allbritton, N
聚合物微流控器件在生物分析中具有巨大的应用潜力。聚二甲基硅氧烷（PDMS）已显示出作为器件快速原型制作基板的前景。尽管如此，在文献中存在分歧的能力，PDMS支持电渗流（EO）的流动和稳定性，随着时间的推移。我们证明，在低离子强度的溶液中接近中性的pH值，氧化的PDMS有四倍更大的EO流动性（亩（EO））相比，原生PDMS。无论使用玻璃还是PDMS作为形成通道一侧的支撑物，都保持较大的mu（eo）。只要通道填充有水溶液，就保持这种增强的mu（eo）。当通道暴露于空气时，迁移率降低了2倍，半衰期为9小时。暴露于空气的氧化PDMS的EO性能通过暴露于强碱而再生。高离子强度，中性pH缓冲液与活的真核细胞相容，减少了氧化的PDMS装置中的EO流量比在原生PDMS装置中更大的程度。对于利用完整和活细胞的分析，PDMS的氧化可能不是显著增加mu（eo）的有效策略。(C)2001爱思唯尔科技有限公司。保留所有权利。
Microfluidic devices fabricated from polymers exhibit great potential in biological analyses. Poly(dimethylsiloxane) (PDMS) has shown promise as a substrate for rapid prototyping of devices. Despite this, disagreement exists in the literature as to the ability of PDMS to support electroosmotic (EO) flow and the stability of that flow over time. We demonstrate that in low ionic strength solutions near neutral in pH, oxidized PDMS had a four-fold greater EO mobility (mu (eo)) compared to native PDMS. The greater mu (eo) was maintained irrespective of whether glass or PDMS was used as a support forming one side of the channel, This enhanced mu (eo) was preserved as long as the channels were filled with an aqueous solution. Upon exposure of the channels to air, the mobility decreased by a factor of two with a half-life of 9 h. The EO properties of the air-exposed, oxidized PDMS were regenerated by exposure to strong base. High ionic strength, neutral in pH buffers compatible with living eukaryotic cells diminished the EO flow in the oxidized PDMS devices to a much greater extent than in the native PDMS devices. For analyses utilizing intact and living cells, oxidation of PDMS may not be an effective strategy to substantially increase the mu (eo). (C) 2001 Elsevier Science B.V. All rights reserved.