The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation
The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation
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DOI：
10.1093/nar/gkg372
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发表时间：
2003-05-15
影响因子：
14.9
通讯作者：
Stewart, AF
中科院分区：
文献类型：
--
作者：
Schaft, D;Roguev, A;Stewart, AF
现有证据表明，酿酒酵母的组蛋白3赖氨酸36甲基转移酶SET2是一种转录抑制因子。在这里，我们通过五条主要的证据表明SET2参与了转录延伸。首先，RNAP II全酶的大部分亚基（如果不是全部的话）与SET2共同纯化。其次，所有共纯化RNAP II亚基RPO21在c -末端结构域（CTD）尾部的5号和2号丝氨酸位点被磷酸化，表明SET2关联是RNAP II的延长型或SSN3抑制型（或两者都有）特异性的。第三，在没有ssn3的情况下，SET2与CTD磷酸化的RPO21的关联仍然存在。第四，在缺少ssn3的情况下，gal1产生mRNA需要SET2。第五，在诱导转录之后，而不是在诱导转录之前，通过体内交联在gal1上检测到SET2。同样，SET2在物理上与pdr5的转录区域相关，但在gal1或pdr5启动子区域未检测到。由于SET2也是一种组蛋白甲基转移酶，这些结果表明组蛋白3赖氨酸36甲基化在转录延伸中起作用。
Existing evidence indicates that SET2, the histone 3 lysine 36 methyltransferase of Saccharomyces cerevisiae, is a transcriptional repressor. Here we show by five main lines of evidence that SET2 is involved in transcriptional elongation. First, most, if not all, subunits of the RNAP II holoenzyme co-purify with SET2. Second, all of the co-purifying RNAP II subunit, RPO21, was phosphorylated at serines 5 and 2 of the C-terminal domain (CTD) tail, indicating that the SET2 association is specific to either the elongating or SSN3 repressed forms (or both) of RNAP II. Third, the association of SET2 with CTD phosphorylated RPO21 remained in the absence of ssn3. Fourth, in the absence of ssn3, mRNA production from gal1 required SET2. Fifth, SET2 was detected on gal1 by in vivo crosslinking after, but not before, the induction of transcription. Similarly, SET2 physically associated with the transcribed region of pdr5 but was not detected on gal1 or pdr5 promoter regions. Since SET2 is also a histone methyltransferase, these results suggest a role for histone 3 lysine 36 methylation in transcriptional elongation.