Collaborative evaluation of optimal antifungal susceptibility testing conditions for dermatophytes
Collaborative evaluation of optimal antifungal susceptibility testing conditions for dermatophytes
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DOI：
10.1128/jcm.40.11.3999-4003.2002
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发表时间：
2002-11-01
影响因子：
9.4
通讯作者：
Guarro, J
中科院分区：
文献类型：
--
作者：
Fernández-Torres, B;Cabañes, FJ;Guarro, J
通过多中心研究确定了皮肤癣菌抗真菌药敏试验的最佳条件。在3个中心对60株皮肤真菌进行了克曲康唑、伊曲康唑和特比萘芬的肉汤微量稀释mic测定。分析接种量(约10（3)和10(4)CFU/ml）、培养时间（3、7和14天）、MIC测定终点标准（完全[MIC-0]和明显[MIC-2]生长抑制）和培养温度（28和37℃）对实验室内和实验室间一致性的影响。确定的最佳检测条件为接种量为10(4)CFU/ml，培养温度为28℃，培养时间为7 d， mic为0。
A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 10(3) and 10(4) CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37degreesC) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 10(4) CFU/ml, a temperature of incubation of 28degreesC, an incubation period of 7 days, and MIC-0.