Evidence for further heterogeneity of the receptors for neuropeptide-Y and peptide-YY in tumor cell lines derived from neural crest.
Evidence for further heterogeneity of the receptors for neuropeptide-Y and peptide-YY in tumor cell lines derived from neural crest.
复制标题
神经嵴来源的肿瘤细胞系中神经肽-Y 和肽-YY 受体进一步异质性的证据。
DOI：
10.1210/endo.131.5.1330489
复制
发表时间：
1992
期刊：
影响因子：
4.8
通讯作者：
Masato Kasuga
中科院分区：
文献类型：
--
作者：
A. Inui;K. Sano;M. Miura;Y. Hirosue;M. Nakajima;M. Okita;S. Baba;Masato Kasuga
用[125 I]Bolton-Hunter-NPY、[125 I]PYY和各种形式的单碘化NPY和PYY，通过配体结合和交联技术，研究了16种人和啮齿动物神经嵴肿瘤细胞系中神经肽Y（NPY）和肽YY（PYY）受体的表达和结构。虽然在大多数肿瘤细胞中观察到NPY结合位点，但仅在人神经母细胞瘤细胞系SMS-MSN、SMS-KAN、SK-N-MC和MC-IXC以及人尤文肉瘤细胞系SK-ES上发现PYY结合位点。这些细胞系上的NPY/PYY受体的差异标记表明，NPY/PYY受体比先前描述的Y1、Y2和Y3受体亚型更异质。交联研究表明，NPY/PYY的Y1和Y2受体在结构上是不同的（mol wt，分别为70和50千道尔顿），并且70和50千道尔顿受体蛋白在某些肿瘤细胞系中共表达。这可以至少部分解释为什么细胞系显示出对Y1/Y2分类的相对特异性，观察到C-末端片段和Y1特异性类似物对NPY/PYY与膜受体结合的抑制。总的来说，本研究表明，进一步的异质性的NPY/PYY受体和存在的多种受体蛋白在肿瘤细胞系中来自神经嵴。
The expression and structure of the receptors for neuropeptide-Y (NPY) and peptide-YY (PYY) were studied in 16 human and rodent tumor cell lines derived from the neural crest by ligand binding and cross-linking techniques using [125I]Bolton-Hunter-NPY, [125I]PYY, and various forms of monoiodinated NPY and PYY. Although NPY-binding sites were observed in most of the tumor cells, PYY-binding sites were found only on the human neuroblastoma cell lines SMS-MSN, SMS-KAN, SK-N-MC, and MC-IXC and the human Ewing's sarcoma cell line SK-ES. The differential labeling of the NPY/PYY receptors on these cell lines suggests that the NPY/PYY receptors are more heterogeneous than previously described as the Y1, Y2, and Y3 receptor subtypes. Cross-linking studies demonstrate that the Y1 and Y2 receptors for NPY/PYY are structurally different (mol wt, 70 and 50 kilodaltons, respectively) and that the 70- and 50-kilodalton receptor proteins are coexpressed in certain tumor cell lines. This could explain at least in part why cell lines show a relative specificity for Y1/Y2 classification, observed as the inhibition by both C-terminal fragments and Y1-specific analogs on the NPY/PYY binding to membrane receptors. Collectively, the present study suggests further heterogeneity of the NPY/PYY receptors and the existence of multiple receptor proteins in the tumor cell lines derived from the neural crest.
肽-YY 和神经肽-Y 抑制大鼠小肠中血管活性肠肽刺激的腺苷 3,5-一磷酸产生：肽与肽-YY 偏好受体相互作用的结构要求。
影响因子：
4.8
作者：
Servin,AL;Rouyer-Fessard,C;Balasubramaniam,A;SaintPierre,S;Laburthe,M
通讯作者：
Laburthe,M
神经肽 Y。与大鼠肠侧基底膜的差异结合。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nguyen,TD;Heintz,GG;Kaiser,LM;Staley,CA;Taylor,IL
通讯作者：
Taylor,IL