Essentiality of sterol synthesis genes in the planctomycete bacterium Gemmata obscuriglobus
Essentiality of sterol synthesis genes in the planctomycete bacterium Gemmata obscuriglobus
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DOI：
10.1038/s41467-019-10983-7
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发表时间：
2019-07
影响因子：
16.6
通讯作者：
Elena Rivas-Marín;Sean Stettner;Ekaterina Y. Gottshall;Carlos Santana-Molina;M. Helling;F. Basile;N. Ward;D. Devos
中科院分区：
文献类型：
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作者：
Elena Rivas-Marín;Sean Stettner;Ekaterina Y. Gottshall;Carlos Santana-Molina;M. Helling;F. Basile;N. Ward;D. Devos
甾体类和类霍帕酸是化学和结构相关的脂质，主要存在于真核生物和细菌细胞膜中。很少有细菌产生甾醇的报道，这种异常最初被归因于真核生物的横向基因转移（LGT）。此外，甾醇在这些细菌中的功能尚不清楚，甾醇和hopanoids之间的功能重叠仍然不清楚。Gemmata obscuriglobus是一种来自浮游菌门的细菌，与其产生hopanoids的亲戚相反，它合成甾醇。在这里，我们表明，甾醇是必不可少的生长的G。obscuriglobus，并且甾醇耗尽导致异常的膜结构和出芽细胞分裂中的缺陷。这篇关于原核生物中甾醇的重要性的报道推进了我们对甾醇分布和功能的理解，并为追求进化细胞生物学中的基本问题提供了基础。
Sterols and hopanoids are chemically and structurally related lipids mostly found in eukaryotic and bacterial cell membranes. Few bacterial species have been reported to produce sterols and this anomaly had originally been ascribed to lateral gene transfer (LGT) from eukaryotes. In addition, the functions of sterols in these bacteria are unknown and the functional overlap between sterols and hopanoids is still unclear.Gemmata obscuriglobusis a bacterium from thePlanctomycetesphylum that synthesizes sterols, in contrast to its hopanoid-producing relatives. Here we show that sterols are essential for growth ofG. obscuriglobus, and that sterol depletion leads to aberrant membrane structures and defects in budding cell division. This report of sterol essentiality in a prokaryotic species advances our understanding of sterol distribution and function, and provides a foundation to pursue fundamental questions in evolutionary cell biology.