THE IMPORTANCE OF SENSITIVE DETECTION OF MALARIA PARASITES IN THE HUMAN AND INSECT HOSTS IN EPIDEMIOLOGIC STUDIES, AS SHOWN BY THE ANALYSIS OF FIELD SAMPLES FROM GUINEA-BISSAU
THE IMPORTANCE OF SENSITIVE DETECTION OF MALARIA PARASITES IN THE HUMAN AND INSECT HOSTS IN EPIDEMIOLOGIC STUDIES, AS SHOWN BY THE ANALYSIS OF FIELD SAMPLES FROM GUINEA-BISSAU
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DOI：
10.1016/0035-9203(93)90274-t
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发表时间：
1993-11-01
影响因子：
2.2
通讯作者：
DOROSARIO, VE
中科院分区：
文献类型：
--
作者：
SNOUNOU, G;PINHEIRO, L;DOROSARIO, VE
一种基于聚合酶链式反应 (PCR) 的高灵敏度检测和鉴定人类疟原虫的方法被应用于从几内亚比绍一个村庄获得的血液和蚊子样本。通过显微镜检查发现，寄生虫在人群中的流行率被大大低估了。特别是，仅通过 PCR 检测就发现三日疟原虫和卵形疟原虫寄生虫的高发病率。初步证据表明，该村内三日疟原虫感染的分布是非随机的。对感染蚊媒的寄生虫种类的分析支持了这一点。讨论了这些结果对流行病学调查的设计和解释的影响。
A method based on the polymerase chain reaction (PCR) for highly sensitive detection and identification of human malaria parasites was applied to blood and mosquito samples obtained from a village in Guinea Bissau. The prevalence of parasites in the human population was shown to be greatly underestimated by microscopical examination. In particular, a high incidence of Plasmodium malariae and P. ovale parasites was revealed only by the PCR assay. Preliminary evidence was obtained to show that the distribution of P. malariae infections within the village was non-random. This was supported by analysis of the parasite species infecting the mosquito vector. The implication of these results for the design and interpretation of epidemiological surveys is discussed.