Do b-ions occur from vibrational excitation upon H-desorption in electron capture dissociation?
Do b-ions occur from vibrational excitation upon H-desorption in electron capture dissociation?
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DOI：
10.1002/rcm.2932
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Schmidt, Marcel
中科院分区：
文献类型：
--
作者：
Haselmann, Kim F.;Schmidt, Marcel
电子捕获解离(ECD)最近被证明在某些情况下可以产生丰富的N末端b离子多肽片段。这些产物离子通常只有在通过振动激发发生激活时才能观察到，如碰撞诱导解离(CID)。在这里，我们证明了合成肽ECD光谱中b离子的出现与低气相碱度相关，并且观察到的b离子碎片是N-末端产物。此外，所有含有b离子的ECD光谱也都有大量的氢和氨从电荷还原物种中损失。版权所有(C)2007 John Wiley&Sons，Ltd.
Electron capture dissociation (ECD) has recently been shown in some cases to produce abundant N-terminal b-ion peptide fragments. These product ions are usually only observed when activation occurs via vibrational excitation as in collision-induced dissociation (CID). Here, we show that occurrence of b-ions in the ECD spectra of synthetic peptides are correlated with low gas-phase basicity and that the observed b-ion fragments are N-terminal products. Furthermore, all ECD spectra containing b-ions also had abundant losses of hydrogen and ammonia from the charge-reduced species. Copyright (c) 2007 John Wiley & Sons, Ltd.