Follicle-Stimulating Hormone Accelerates Mouse Oocyte Development In Vivo
Follicle-Stimulating Hormone Accelerates Mouse Oocyte Development In Vivo
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DOI：
10.1095/biolreprod.112.099929
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发表时间：
2012-07-01
影响因子：
3.6
通讯作者：
Clarke, Hugh J.
中科院分区：
文献类型：
--
作者：
Demeestere, Isabelle;Streiff, Agathe K.;Clarke, Hugh J.
在卵泡发生过程中，卵母细胞与其相邻的体细胞相互依赖地生长并获得发育能力。卵泡刺激素(FSH)在体细胞分化中起着重要的作用，但其在卵母细胞发育中的作用尚不清楚。我们在这里报道，FSHB(-/-)小鼠的卵母细胞不能产生FSH，但其生长速度和大小与野生型小鼠相同。与此一致的是，FSHB(-/-)小鼠的颗粒细胞表达正常数量的编码Kit配体的mRNA，这与卵母细胞的生长有关。FSHB(-/-)小鼠的卵母细胞也积累了正常数量的细胞周期蛋白B1和CDK1蛋白以及线粒体DNA。此外，它们还获得了在体外完成减数分裂成熟的能力，并经历了从非包围核仁到包围核仁的过渡。然而，这些卵母细胞发育后期的事件明显延迟。FSHB(-/-)小鼠卵母细胞体外成熟受精后，仅有少量胚胎进入囊胚期。在体外成熟前48小时给予马绒毛膜促性腺激素，可增加随后获得的囊胚数。这些结果表明，卵泡刺激素并不是卵母细胞在体内发育所必需的，但在它的存在下，这一过程发生得更快。我们认为，FSH可能协调卵泡生殖线和体细胞的发育，确保排卵释放出具有发育能力的卵子。
During folliculogenesis, oocytes grow and acquire developmental competence in a mutually dependent relationship with their adjacent somatic cells. Follicle-stimulating hormone (FSH) plays an essential and well-established role in the differentiation of somatic follicular cells, but its function in the development of the oocyte has still not been elucidated. We report here that oocytes of Fshb(-/-) mice, which cannot produce FSH, grow at the same rate and reach the same size as those of wild-type mice. Consistent with this observation, the granulosa cells of Fshb(-/-) mice express the normal quantity of mRNA encoding Kit ligand, which has been implicated in oocyte growth. Oocytes of Fshb(-/-) mice also accumulate normal quantities of cyclin B1 and CDK1 proteins and mitochondrial DNA. Moreover, they acquire the ability to complete meiotic maturation in vitro and undergo transition from non-surrounded nucleolus to surrounded nucleolus. However, these events of late oocyte development are significantly delayed. Following in vitro maturation and fertilization, only a small number of embryos derived from oocytes of Fshb(-/-) mice reach the blastocyst stage. Administration of equine chorionic gonadotropin, which provides FSH activity, 48 h before in vitro maturation increases the number of blastocysts obtained subsequently. These results indicate that FSH is not absolutely required for oocyte development in vivo but that this process occurs more rapidly in its presence. We suggest that FSH may coordinate the development of the germline and somatic compartments of the follicle, ensuring that ovulation releases a developmentally competent egg.