EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 (HIV-1) GAG ANTIGENS ON THE SURFACE OF A CELL-LINE PERSISTENTLY INFECTED WITH HIV-1 THAT HIGHLY EXPRESSES HIV-1 ANTIGENS
EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 (HIV-1) GAG ANTIGENS ON THE SURFACE OF A CELL-LINE PERSISTENTLY INFECTED WITH HIV-1 THAT HIGHLY EXPRESSES HIV-1 ANTIGENS
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DOI：
10.1016/0042-6822(89)90431-5
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发表时间：
1989-06-01
期刊：
影响因子：
3.7
通讯作者：
KATO, S
中科院分区：
文献类型：
--
作者：
IKUTA, K;MORITA, C;KATO, S
最近分离出持续感染人类免疫缺陷病毒1型（HIV-1）的MT-4细胞（MT-4/HIV-1）（K.伊库塔角Morita，M. Nakai，N. Yamamota和S.日本，加藤。（Gann），79，418-423，1988）。利用该MT-4/HIV-1细胞系分离出产生抗HIV-1 gag p24和p18以及pol逆转录酶（RT）的单克隆抗体（MoAbs）的小鼠杂交瘤细胞克隆，并通过免疫荧光（IF）试验筛选MoAbs的产生。用这些单克隆抗体对丙酮固定的细胞进行间接干扰试验，发现MT-4/HIV-1细胞的干扰强度高于其他HIV-1感染细胞，如MOLT-4/HIV-1、HL-60/HIV-1和U937/HIV-1细胞。用这些单克隆抗体通过IF和放射免疫技术检测的HIV gag p24和p18抗原的细胞表面表达显示，p24和p18抗原分别在MT-4/HIV-1细胞的细胞表面上强烈表达和在MOLT-4/HIV-1细胞的细胞表面上微弱表达。然而，在本研究中分离的单克隆抗体未能检测MT-4/HIV-1细胞表面上的polRT抗原。这些结果表明，gag p24和p18抗原至少部分地在HIV感染细胞的表面上表达。
MT-4 cells persistently infected with human immunodeficiency virus type 1 (HIV-1) (MT-4/HIV-1) were recently isolated (K. Ikuta, C. Morita, M. Nakai, N. Yamamota, and S. Kato, Japan. J. Cancer Res. (Gann), 79, 418-423, 1988). Mouse hybridoma cell clones producing monoclonal antibodies (MoAbs) to HIV-1 gag p24 and p18, and pol reverse transcriptase (RT) were isolated by using this MT-4/HIV-1 cell line for the screening of MoAb production by the immunofluorescence (IF) test. By indirect IF tests of acetone-fixed cells with these MoAbs, the IF intensities in MT-4/HIV-1 cells were found to be higher than those in the other HIV-1 infected cells, such as MOLT-4/HIV-1, HL-60/HIV-1, and U937/HIV-1 cells. Cell surface expression of the HIV gag p24 and p18 antigens examined by IF and radioimmune techniques with these MoAbs revealed the p24 and p18 antigens to be expressed strongly on the cell surface of MT-4/HIV-1 cells and faintly on the cell surface of MOLT-4/HIV-1 cells, respectively. However, monoclonal antibody isolated in the present study failed to detect polRT antigen on the surface of MT-4/HIV-1 cells. These results indicate that the gag p24 and p18 antigens are expressed, at least in part, on the surface of HIV--infected cells.