DIFFERENTIAL LOCALIZATION OF NMDA AND AMPA RECEPTOR SUBUNITS IN THE LATERAL AND BASAL NUCLEI OF THE AMYGDALA - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
DIFFERENTIAL LOCALIZATION OF NMDA AND AMPA RECEPTOR SUBUNITS IN THE LATERAL AND BASAL NUCLEI OF THE AMYGDALA - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1002/cne.903620106
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发表时间：
1995-11-06
影响因子：
2.5
通讯作者：
LEDOUX, JE
中科院分区：
文献类型：
--
作者：
FARB, CR;AOKI, C;LEDOUX, JE
解剖学和生理学研究表明，氨基酸L-谷氨酸是杏仁核的感觉传入通路和涉及外侧核和基底核的杏仁核内回路中的兴奋性递质。区域，细胞，和亚细胞免疫细胞化学定位的N-甲基-D-天冬氨酸（NMDA）和L-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA），两个主要类别的谷氨酸受体，在杏仁核的这些地区进行了检查。使用针对NMDA受体R1亚基的单克隆抗体和多克隆抗血清。每种免疫试剂产生不同的分布核周和神经纤毛染色。树突状免疫反应主要定位于不对称（兴奋性）突触连接，主要是在棘，与传统的组织和功能的NMDA受体的观点一致。而抗NMDAR 1抗血清产生稀疏的突触前轴突末端标记和广泛的神经胶质标记，抗NMDAR 1抗体标记相当少的神经胶质和更多的突触前轴突末端。标记的突触前末梢形成不对称和对称的突触，表明兴奋性和抑制性传递的突触前调节。AMPA受体不同亚基（GluR 1、GluR 2/3和GluR 4）的免疫反应性在神经元群体中分布独特，某些受体亚基对某些细胞类型具有特异性。GluR 1和Glu 2/3的免疫反应性主要定位于树突轴，并且由于树突近端部分的重标记而比GluR 4更广泛。GluR 4免疫反应性的分布是类似的NMDAR 1：GluR 4被认为是在突触前末梢，神经胶质细胞，树突和主要定位于棘。在缺乏GluR 2的情况下GluR 4的突触前定位表明谷氨酸介导的突触前Ca++浓度调节。这些数据增加了我们对杏仁核突触前和突触后传递机制和突触可塑性的形态学基础的理解。(C)1995年Wiley-Liss，Inc.
Anatomical and physiological studies indicate that the amino acid L-glutamate is the excitatory transmitter in sensory afferent pathways to the amygdala and in intraamygdala circuits involving the lateral and basal nuclei. The regional, cellular, and subcellular immunocytochemical localizations of N-methyl-D-aspartate (NMDA) and L-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), two major classes of glutamate receptors, were examined in these areas of the amygdala. A monoclonal antibody and a polyclonal antiserum directed against the R1 subunit of the NMDA receptor were used. Each immunoreagent produced distinct distributions of perikaryal and neuropilar staining. Dendritic immunoreactivity was localized primarily to asymmetric (excitatory) synaptic junctions, mostly on spines, consistent with the conventional view of the organization and function of NMDA receptors. Whereas the anti-NMDAR1 antiserum produced sparse presynaptic axon terminal labeling and extensive glial labeling, the anti-NMDAR1 antibody labeled considerably fewer glia and many more presynaptic axon terminals. Labeled presynaptic terminals formed asymmetric and symmetric synapses, suggesting presynaptic regulation of both excitatory and inhibitory transmission. Immunoreactivity for different subunits of the AMPA receptor (GluR1, GluR2/3, and GLuR4) was uniquely distributed across neuronal populations, and some receptor subunits were specific to certain cell types. Immunoreactivity for GluR1 and Glu2/3 was predominately localized to dendritic shafts and was more extensive than that of GluR4 due to heavy labeling of proximal portions of dendrites. The distribution of GluR4 immunoreactivity was similar to NMDAR1: GluR4 was seen in presynaptic terminals, glia, and dendrites and was primarily localized to spines. The presynaptic localization of GluR4 in the absence of GluR2 suggests glutamate-mediated modulation of presynaptic Ca++ concentrations. These data add to our understanding of the morphological basis of pre- and postsynaptic transmission mechanisms and synaptic plasticity in the amygdala. (C) 1995 Wiley-Liss, Inc.