Tomography of DNA tiles influences the kinetics of surface-mediated DNA self-assembly
Tomography of DNA tiles influences the kinetics of surface-mediated DNA self-assembly
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DNA 瓦片的断层扫描影响表面介导的 DNA 自组装的动力学
DOI：
10.1016/j.bpj.2022.07.025
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发表时间：
2022
影响因子：
3.4
通讯作者：
Mao, Chengde
中科院分区：
文献类型：
--
作者：
Zhang, Cuizheng;Paluzzi, Victoria E.;Mao, Chengde
本文研究了挤压发夹结构对DNA分子在固体表面二维自组装的影响。发夹结构通常用作DNA纳米结构的断层扫描标记，用于原子力显微镜成像。在这项研究中，我们发现发夹发挥了更积极的作用。它们调节DNA瓦片在固体表面上的吸附，从而改变瓦片在固体表面上的组装动力学。基于这一发现，我们能够通过改变DNA瓦片上的发夹位置来促进或减缓DNA在表面上的自组装。这些知识的获得将有助于未来的DNA表面自组装的设计。
This manuscript studies the impact of extruding hairpins on two-dimensional self-assembly of DNA tiles on solid surface. Hairpins are commonly used as tomographic markers in DNA nanostructures for atomic force microscopy imaging. In this study, we have discovered that hairpins play a more active role. They modulate the adsorption of the DNA tiles onto the solid surface, thus changing the tile assembly kinetics on the solid surface. Based on this discovery, we were able to promote or slow down DNA self-assembly on the surface by changing the hairpin locations on the DNA tiles. This knowledge gained will be helpful for the future design of DNA self-assembly on surface.