Skeletal Muscle Regeneration in Zebrafish.
Skeletal Muscle Regeneration in Zebrafish.
复制标题
斑马鱼的骨骼肌再生。
DOI：
10.1007/978-1-0716-3036-5_17
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Pipalia TG
中科院分区：
文献类型：
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作者：
Pipalia TG
肌肉再生模型揭示了炎症、伤口清除和干细胞导向的损伤修复机制，从而指导治疗。虽然肌肉修复的研究在啮齿类动物中最先进，但斑马鱼正在成为一种额外的模式生物，具有遗传和视觉优势。各种肌肉损伤方案(包括化学和物理)已经公布。在这里，我们描述了简单、廉价、精确、适用和有效的斑马鱼幼体骨骼肌再生模型的两个阶段的损伤方案和分析方法。我们展示了肌肉损伤、肌肉干细胞、免疫细胞内移和纤维再生在个体幼虫中如何在较长的时间内被监测的例子。这样的分析有可能极大地增强理解，因为它减少了对受到不可避免的可变伤口刺激的个体进行平均再生反应的需要。
Muscle regeneration models have revealed mechanisms of inflammation, wound clearance, and stem cell–directed repair of damage, thereby informing therapy. Whereas studies of muscle repair are most advanced in rodents, the zebrafish is emerging as an additional model organism with genetic and optical advantages. Various muscle wounding protocols (both chemical and physical) have been published. Here we describe simple, cheap, precise, adaptable, and effective wounding protocols and analysis methods for two stages of a larval zebrafish skeletal muscle regeneration model. We show examples of how muscle damage, ingression of muscle stem cells, immune cells, and regeneration of fibers can be monitored over an extended timecourse in individual larvae. Such analyses have the potential to greatly enhance understanding, by reducing the need to average regeneration responses across individuals subjected to an unavoidably variable wound stimulus.