DIHYDROLIPOIC ACID PROTECTS PANCREATIC-ISLET CELLS FROM INFLAMMATORY ATTACK
DIHYDROLIPOIC ACID PROTECTS PANCREATIC-ISLET CELLS FROM INFLAMMATORY ATTACK
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DOI：
10.1007/bf02027215
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
KOLB, H
中科院分区：
文献类型：
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作者：
BURKART, V;KOIKE, T;KOLB, H
胰岛细胞炎症的体外模型是通过活化的巨噬细胞或内皮酶黄嘌呤氧化酶释放的氧自由基裂解分离的胰岛细胞。二氢硫辛酸通过不同的作用方式保护两个系统中的胰岛细胞。巨噬细胞与硫辛酸预孵育 2 小时可抑制一氧化氮介导的巨噬细胞对胰岛细胞的细胞毒性。同样，2 小时的预孵育足以保护胰岛细胞免受酶促产生的氧自由基的侵害。通过化学发光测定发现二氢硫辛酸可以直接清除此类自由基。在巨噬细胞中，二氢硫辛酸抑制亚硝酸盐的产生，作为一氧化氮释放的量度。这些结果表明二氢硫辛酸是一种抗炎剂，同时干扰炎症巨噬细胞释放一氧化氮并保护靶细胞免受氧自由基的攻击。
In vitro models of pancreatic islet cell inflammation are the lysis of isolated islet cells by activated macrophages or by oxygen radicals released by the endothelial enzyme xanthine oxidase. Dihydrolipoic acid protected islet cells in both systems by different modes of action. Macrophage cytotoxicity towards islet cells, which is nitric-oxide-mediated, was suppressed by 2 h of preincubation of macrophages with lipoic acid. Similarly, 2 h of preincubation sufficed to protect islet cells against enzymatically produced oxygen radicals. Dihydrolipoic acid was found by chemiluminescence assay to scavenge directly such radicals. In macrophages dihydrolipoic acid suppressed the production of nitrite as a measure of nitric oxide release. These results suggest that dihydrolipoic acid is an anti-inflammatory agent which at the same time interferes with nitric oxide release from inflammatory macrophages and protects target cells from oxygen radical attack.