CHARACTERIZATION, MAPPING, AND EXPRESSION OF THE HUMAN CERULOPLASMIN GENE
CHARACTERIZATION, MAPPING, AND EXPRESSION OF THE HUMAN CERULOPLASMIN GENE
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DOI：
10.1073/pnas.83.10.3257
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发表时间：
1986-05-01
影响因子：
11.1
通讯作者：
BOWMAN, BH
中科院分区：
文献类型：
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作者：
YANG, F;NAYLOR, SL;BOWMAN, BH
铜蓝蛋白是一种存在于脊椎动物血浆中的铜结合蛋白。它是基因内三次复制的产物，由三个同源结构域组成。利用已发表的氨基酸序列构建的寡核苷酸探针对编码人类CP的cDNA克隆进行了鉴定。CP-1和CP-2两个克隆的不同之处在于分别存在或不存在一个推断的4个氨基酸序列。这两个克隆提供了81%的CP编码序列。CP编码序列的三个结构域的核苷酸比较表明，CP编码序列的内部结构域同源性在50.1% ~ 56%之间。将CP-2 cDNA的核苷酸序列与人类同源蛋白凝血因子VIII的核苷酸序列进行比较，发现其整体相同度为48%。通过体细胞杂交分析，将CP基因定位到人第3染色体上，原位杂交定位到第3q25染色体上；然而，与其他染色体上DNA的杂交位点表明，人类基因组中存在额外的cp样DNA序列。用Pst酶切人DNA后，用CP cDNA检测DNA多态性。用原位组织杂交法检测人肝脏、巨噬细胞和淋巴细胞中CP mRNA的表达。
Ceruloplasmin (CP) is a copper-binding protein in vertebrate plasma. It is the product of an intragenic triplication and is composed of three homologous domains. Oligonucleotide probes constructed according to published amino acid sequences were used to identify cDNA clones encoding human CP. Two clones, CP-1 and CP-2, differed from each other by the presence or absence, respectively, of a deduced sequence of four amino acids. The two clones provided 81% of the sequence encoding CP. Comparison of the nucleotides of the three domains of the CP coding sequence revealed internal domain homology with identity of sequences ranging from 50.1% to 56%. The nucleotide sequence of CP-2 cDNA was compared to that of a homologous human protein, clotting factor VIII, and was found to be 48% identical overall. The CP gene was mapped to human chromosome 3 by somatic-cell-hybrid analysis and to 3q25 by in situ hybridization; however, sites of hybridization to DNA on other chromosomal sites suggested additional CP-like DNA sequences in the human genome. A DNA polymorphism was detected with CP cDNA after endonuclease digestion of human DNA by Pst I. CP mRNA was detected in human liver, macrophages, and lymphocytes by in situ histohybridization.