A scalable phenotyping approach for female floral organ development and senescence in the absence of pollination in wheat.
A scalable phenotyping approach for female floral organ development and senescence in the absence of pollination in wheat.
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DOI：
10.1242/dev.200889
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发表时间：
2022-09-15
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影响因子：
--
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在没有授粉的情况下，雌性生殖器官衰老，导致花的生殖潜力不可挽回地丧失，这直接影响种子结实。在小麦(Triticum Aestivum)等自花授粉作物中，未授粉心皮的花后活力一直被忽视，尽管它对杂交种子生产系统很重要。为了提高我们在没有授粉的情况下心皮发育的知识，我们创造了一种高通量的表型方法来量化柱头和子房的形态。我们证明了使用光学显微镜成像和机器学习的方法对于使用新鲜和固定样本分析田间生长的植物的花器官特征的适用性。我们发现，未授粉的心皮经历了一个明确的初始生长阶段，然后是柱头面积达到最大和子房径向扩张减慢的高峰期，最后是退化阶段。这些发育动态在多年间是一致的，可用于不育品种的分类。这种表型方法为研究心皮发育提供了一种新的工具，我们希望这将促进对小麦雌性育性的研究。摘要：一种新的小麦柱头和子房发育的自动化表型方法突出了遗传变异的潜力，这可能有助于杂交育种。
In the absence of pollination, female reproductive organs senesce, leading to an irrevocable loss in the reproductive potential of the flower, which directly affects seed set. In self-pollinating crops like wheat (Triticum aestivum), the post-anthesis viability of unpollinated carpels has been overlooked, despite its importance for hybrid seed production systems. To advance our knowledge of carpel development in the absence of pollination, we created a high-throughput phenotyping approach to quantify stigma and ovary morphology. We demonstrate the suitability of the approach, which uses light-microscopy imaging and machine learning, for the analysis of floral organ traits in field-grown plants using fresh and fixed samples. We show that the unpollinated carpel undergoes a well-defined initial growth phase, followed by a peak phase in which stigma area reaches its maximum and the radial expansion of the ovary slows, and a final deterioration phase. These developmental dynamics were consistent across years and could be used to classify male-sterile cultivars. This phenotyping approach provides a new tool for examining carpel development, which we hope will advance research into female fertility of wheat. Summary: A new automated phenotyping method for stigma and ovary development in wheat highlights the potential for genetic variation that could benefit hybrid breeding.
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影响因子：
2.3
作者：
BASSETTI, P;WESTGATE, ME
通讯作者：
WESTGATE, ME
影响因子：
6.9
作者：
GONZALEZ, MV;COQUE, M;HERRERO, M
通讯作者：
HERRERO, M
DOI：
10.1071/ea9660175
发表时间：
1966-01-01
期刊：
AUSTRALIAN J EXP AGR ANIM HUSB
影响因子：
--
作者：
IMRIE, B. C.
通讯作者：
IMRIE, B. C.
影响因子：
46.9
作者：
Bray, Nicolas L.;Pimentel, Harold;Pachter, Lior
通讯作者：
Pachter, Lior
影响因子：
4.8
作者：
DICE, LR
通讯作者：
DICE, LR