Immunochemical studies on monoclonal antibodies to stearyl-isomaltotetraose from C58/J and a C57BL/10 nude mouse.
Immunochemical studies on monoclonal antibodies to stearyl-isomaltotetraose from C58/J and a C57BL/10 nude mouse.
复制标题
来自 C58/J 和 C57BL/10 裸鼠的硬脂酰异麦芽四糖单克隆抗体的免疫化学研究。
DOI：
10.1016/0161-5890(87)90174-x
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发表时间：
1987
影响因子：
3.6
通讯作者：
Kabat,EA
中科院分区：
文献类型：
--
作者：
Chen,HT;Makover,SD;Kabat,EA
Six hybridomas, five from C58/J and one from C57BL/10 nu/nu, immunized with stearyl-isomaltotetraose (S-IM4) were established. One produced IgG3, one IgM and four IgA. The specificities and sizes of the antibody combining sites were determined by quantitative precipitin and ELISA quantitative inhibition assays. All cross-react with α(1→6)dextran B512. Their combining sites were complementary to five to seven glucose residues. Association constants of hybridoma antibodies for dextran B512 ranged from 103to 105ml/g, and for isomaltoheptaose (IM7) from 103to 104M−1. Two hybridoma antibodies, one IgA and another IgM derived from different fusions have identical inhibition curves and combining sites, and very close association constants. It will be important to establish whether they have very similar or identical nucleotide sequences in their variable regions. These studies provide further insight into the specificity and repertoire of antidextran combining sites.
